Characterization of Fv-1 gene-product-mediated resistance transfer

Ralph J. Rascati, James A. Otten, Arthur Brown, Julia E. Hannan, Wen-Kuang Yang, Raymond W. Tennant

Research output: Contribution to journalArticle

Abstract

Fv-1 gene-specific resistance to eco tropic murine retro viruses can be transferred to permissive cells with RNA prepared from restrictive cells. The active RNA has a sedimentation coefficient of 20–22S at low ionic strength and multiple regions of activity (5–6S, 10–12S, 24–27S) at high ionic strength. The maximum level of resistance transfer cannot be enhanced with noninhibitory carrier RNA or facilitators of RNA uptake, and the maximum inhibition (40–60%) is independent of the MOI. The low efficiency of resistance transfer is, therefore, probably the result of a fraction of cells resistant to RNA uptake.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalIntervirology
Volume15
Issue number2
DOIs
StatePublished - Jan 1 1981

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RNA
Genes
Osmolar Concentration
Viruses

Keywords

  • Fv-1 locus
  • Gene product
  • Host cell control
  • Retrovirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Rascati, R. J., Otten, J. A., Brown, A., Hannan, J. E., Yang, W-K., & Tennant, R. W. (1981). Characterization of Fv-1 gene-product-mediated resistance transfer. Intervirology, 15(2), 87-96. https://doi.org/10.1159/000149218

Characterization of Fv-1 gene-product-mediated resistance transfer. / Rascati, Ralph J.; Otten, James A.; Brown, Arthur; Hannan, Julia E.; Yang, Wen-Kuang; Tennant, Raymond W.

In: Intervirology, Vol. 15, No. 2, 01.01.1981, p. 87-96.

Research output: Contribution to journalArticle

Rascati, RJ, Otten, JA, Brown, A, Hannan, JE, Yang, W-K & Tennant, RW 1981, 'Characterization of Fv-1 gene-product-mediated resistance transfer', Intervirology, vol. 15, no. 2, pp. 87-96. https://doi.org/10.1159/000149218
Rascati RJ, Otten JA, Brown A, Hannan JE, Yang W-K, Tennant RW. Characterization of Fv-1 gene-product-mediated resistance transfer. Intervirology. 1981 Jan 1;15(2):87-96. https://doi.org/10.1159/000149218
Rascati, Ralph J. ; Otten, James A. ; Brown, Arthur ; Hannan, Julia E. ; Yang, Wen-Kuang ; Tennant, Raymond W. / Characterization of Fv-1 gene-product-mediated resistance transfer. In: Intervirology. 1981 ; Vol. 15, No. 2. pp. 87-96.
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