Characterization of miR-200 family members as blood biomarkers for human and laying hen ovarian cancer

Pui Wah Choi, Abbas Bahrampour, Shu Kay Ng, Sze Kei Liu, Wei Qiu, Fang Xie, Winston Patrick Kuo, Joseph Kwong, Karen H. Hales, Dale B. Hales, Kwong Kwok Wong, Errol R. Norwitz, Chun Kin Chow, Ross S. Berkowitz, Shu Wing Ng

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. We evaluated the levels of family members relative to the internal control miR-103a in ovarian cancer and control blood specimens collected from American and Hong Kong Chinese institutions, as well as from a laying hen spontaneous ovarian cancer model. The levels of miR-200a, miR-200b and miR-200c were significantly elevated in all human cancer versus all control blood samples. Further analyses showed significantly higher miR-200 levels in Chinese control (except miR-429) and cancer (except miR-200a and miR141) samples than their respective American counterparts. Subtype-specific analysis showed that miR-200b had an overall elevated level in serous cancer compared with controls, whereas miR-429 was significantly elevated in clear cell and endometrioid cancer versus controls. MiR-429 was also significantly elevated in cancer versus control in laying hen plasma samples, consistent with the fact that endometrioid tumor is the prevalent type in this species. A neural network model consisting of miR-200a/200b/429/141 showed an area under the curve (AUC) value of 0.904 for American ovarian cancer prediction, whereas a model consisting of miR-200b/200c/429/141 showed an AUC value of 0.901 for Chinese women. Hence, miR-200 is informative as blood biomarkers for both human and laying hen ovarian cancer.

Original languageEnglish (US)
Article number20071
JournalScientific reports
Volume10
Issue number1
DOIs
StatePublished - Dec 2020

ASJC Scopus subject areas

  • General

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