TY - JOUR
T1 - Characterization of the BUD31 gene of Saccharomyces cerevisiae
AU - Masciadri, Barbara
AU - Areces, Liliana B.
AU - Carpinelli, Patrizia
AU - Foiani, Marco
AU - Draetta, Giulio F.
AU - Fiore, Francesca
N1 - Funding Information:
We thank Simona Ronzoni and Ivan Muradore for FACS analysis and sequencing core at IFOM. We gratefully thank Metello Innocenti and Simonetta Piatti for helpful suggestions and critical reading of the manuscript. This work was supported by grants from Associazione Italiana per la Ricerca sul Cancro (AIRC), Consiglio Nazionale delle Ricerche (CNR), Fondazione Italiana per la Ricerca sul Cancro (FIRC), and Telethon to G.F.D. Barbara Masciadri was supported by a fellowship from FIRC (Fondazione Italiana Ricerca sul Cancro).
PY - 2004/8/6
Y1 - 2004/8/6
N2 - A number of genes have been identified in the fully sequenced genome of Saccharomyces cerevisiae that appear to be conserved throughout evolution and the function of which remains poorly understood. In this manuscript we describe the initial characterization of yeast BUD31 gene. cDNA sequences highly related to BUD31 have been identified in human, Xenopus laevis, and Caenorhabditis elegans. With the aim of further understanding its function, we generated a BUD31-null yeast strain and characterized its phenotype: bud31 mutant cells showed severe cytoskeletal abnormalities, with dramatic effects on actin distribution and bud formation. We also proceeded to identify interacting proteins using the tandem affinity-purification method, coupled to mass spectrometry: Bud31p was found in complex with proteins involved in mRNA splicing. We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud31p and its homologs.
AB - A number of genes have been identified in the fully sequenced genome of Saccharomyces cerevisiae that appear to be conserved throughout evolution and the function of which remains poorly understood. In this manuscript we describe the initial characterization of yeast BUD31 gene. cDNA sequences highly related to BUD31 have been identified in human, Xenopus laevis, and Caenorhabditis elegans. With the aim of further understanding its function, we generated a BUD31-null yeast strain and characterized its phenotype: bud31 mutant cells showed severe cytoskeletal abnormalities, with dramatic effects on actin distribution and bud formation. We also proceeded to identify interacting proteins using the tandem affinity-purification method, coupled to mass spectrometry: Bud31p was found in complex with proteins involved in mRNA splicing. We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud31p and its homologs.
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U2 - 10.1016/j.bbrc.2004.05.228
DO - 10.1016/j.bbrc.2004.05.228
M3 - Article
C2 - 15303280
AN - SCOPUS:3142665424
SN - 0006-291X
VL - 320
SP - 1342
EP - 1350
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 4
ER -