Cloning of novel trinucleotide-repeat (CAG) containing genes from mouse brain

S. J. Kim, B. H. Son, J. H. Kang, K. S. Hahm, O. J. Yoo, K. K. Lee

Research output: Contribution to journalArticlepeer-review

Abstract

To identify novel mouse genes with CAG trinucleotide repeats expressed in the mouse brain, Agtl0 cDNA library of mouse brain was screened with a oligonucleotide containing ten repeats of CTG triplet. Of the positives, 11 were selected for plaque purification. The size of the inserts ranged from 0.5 to 2.1 kb. Sequence analysis revealed that eight clones were novel mouse genes. The length of the longest simple CAG repeats ranged from 6 to 25. It was noteworthy that the amino acid sequences of CAG-6 deduced from the nucleotide sequence shared 13 consecutive identical residues at just upstream region of the CTR sequence with the OB-cadherin gene, a member of cadherin family which is highly expressed in osteoblasts and weakly expressed in brain. The nucleotide sequence in this region is 5'-GGCATAGAACTGTTTGAAATC (T in case of OB-cadherin) ACAACAGACTATGAAACA-3'. CAG-14 showed high homology (64%) with the 3'-untranslated region of rat leukocyte common antigen-related (LAR) tyrosine phosphatase receptor. In rat LAR tyrosine phosphatase receptor, the CTR sequence is found in only one of five alternatively spliced transcripts. The presence of CTR in the clone CTG-I.I may imply the expression of the specific transcript in mouse brain as well. Southern blots of the mouse genomic DNA and RT-PCR anlayses proved that the CTR clones were from mouse genes.

Original languageEnglish (US)
Pages (from-to)A1372
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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