Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement

A. Haleem; J. Kurtzberg; G. A. Olsen; A. Rhinehardt-Clark; D. S. Leslie; L. Ray; C. A. Smith; W. P. Peters; B. F. Haynes; R. C. Bast

Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement

A. Haleem, J. Kurtzberg, G. A. Olsen, A. Rhinehardt-Clark, D. S. Leslie, L. Ray, C. A. Smith, W. P. Peters, B. F. Haynes, R. C. Bast

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 μ m 2’ -deoxy coformycin and 500 μM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-form-ing erythroid units, or multipotential erythroid-granulocyte-megakary-ocyte-macrophage colony-forming hematopoietic progenitor cell units.

Original language	English (US)
Pages (from-to)	4608-4612
Number of pages	5
Journal	Cancer Research
Volume	47
Issue number	17
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

Haleem, A., Kurtzberg, J., Olsen, G. A., Rhinehardt-Clark, A., Leslie, D. S., Ray, L., Smith, C. A., Peters, W. P., Haynes, B. F., & Bast, R. C. (1987). Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement. Cancer Research, 47(17), 4608-4612.

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title = "Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2{\textquoteright}-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement",

abstract = "Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 μ m 2{\textquoteright} -deoxy coformycin and 500 μM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-form-ing erythroid units, or multipotential erythroid-granulocyte-megakary-ocyte-macrophage colony-forming hematopoietic progenitor cell units.",

author = "A. Haleem and J. Kurtzberg and Olsen, {G. A.} and A. Rhinehardt-Clark and Leslie, {D. S.} and L. Ray and Smith, {C. A.} and Peters, {W. P.} and Haynes, {B. F.} and Bast, {R. C.}",

year = "1987",

language = "English (US)",

volume = "47",

pages = "4608--4612",

journal = "Cancer Research",

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TY - JOUR

T1 - Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement

AU - Haleem, A.

AU - Kurtzberg, J.

AU - Olsen, G. A.

AU - Rhinehardt-Clark, A.

AU - Leslie, D. S.

AU - Ray, L.

AU - Smith, C. A.

AU - Peters, W. P.

AU - Haynes, B. F.

AU - Bast, R. C.

PY - 1987

Y1 - 1987

N2 - Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 μ m 2’ -deoxy coformycin and 500 μM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-form-ing erythroid units, or multipotential erythroid-granulocyte-megakary-ocyte-macrophage colony-forming hematopoietic progenitor cell units.

AB - Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 μ m 2’ -deoxy coformycin and 500 μM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-form-ing erythroid units, or multipotential erythroid-granulocyte-megakary-ocyte-macrophage colony-forming hematopoietic progenitor cell units.

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AN - SCOPUS:0023221440

SN - 0008-5472

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JO - Cancer Research

JF - Cancer Research

IS - 17

ER -

Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement

Abstract

ASJC Scopus subject areas

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