Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement

A. Haleem, J. Kurtzberg, G. A. Olsen, A. Rhinehardt-Clark, D. S. Leslie, L. Ray, C. A. Smith, W. P. Peters, B. F. Haynes, Robert C Bast Jr

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 μ m 2’ -deoxy coformycin and 500 μM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-form-ing erythroid units, or multipotential erythroid-granulocyte-megakary-ocyte-macrophage colony-forming hematopoietic progenitor cell units.

Original languageEnglish (US)
Pages (from-to)4608-4612
Number of pages5
JournalCancer Research
Volume47
Issue number17
StatePublished - Jan 1 1987

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Pentostatin
T-Cell Lymphoma
Bone Marrow
Monoclonal Antibodies
Coformycin
Granulocyte-Macrophage Progenitor Cells
Hematopoietic Stem Cells
Granulocytes
Macrophages
Rabbits
Antibodies
Growth
2'-deoxyadenosine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement. / Haleem, A.; Kurtzberg, J.; Olsen, G. A.; Rhinehardt-Clark, A.; Leslie, D. S.; Ray, L.; Smith, C. A.; Peters, W. P.; Haynes, B. F.; Bast Jr, Robert C.

In: Cancer Research, Vol. 47, No. 17, 01.01.1987, p. 4608-4612.

Research output: Contribution to journalArticle

Haleem, A, Kurtzberg, J, Olsen, GA, Rhinehardt-Clark, A, Leslie, DS, Ray, L, Smith, CA, Peters, WP, Haynes, BF & Bast Jr, RC 1987, 'Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement', Cancer Research, vol. 47, no. 17, pp. 4608-4612.
Haleem, A. ; Kurtzberg, J. ; Olsen, G. A. ; Rhinehardt-Clark, A. ; Leslie, D. S. ; Ray, L. ; Smith, C. A. ; Peters, W. P. ; Haynes, B. F. ; Bast Jr, Robert C. / Combined Chemoseparation and Immunoseparation of Clonogenic T Lymphoma Cells from Human Bone Marrow Using 2’-Deoxycoformycin, Deoxyadenosine, 3A1 Monoclonal Antibody, and Complement. In: Cancer Research. 1987 ; Vol. 47, No. 17. pp. 4608-4612.
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