Comparative evaluation of two indigenously developed tests for rapid detection of group-A streptococci directly from throat swabs

Rita Gupta, Awdhesh Kalia, Ashok Rattan, Ramesh Kumar, S. K. Gupta

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The efficacy of two indigenously developed rapid tests, latex agglutination and antibody capture assay (a colour immunochromatographic assay) to detect group A Streptococcus pyogenes (GAS) was evaluated in comparison with the traditional culture method ('Gold Standard') in both asymptomatic children (1500) and symptomatic patients (233). Throat swabs were taken in duplicate and rapid tests performed on one swab and culture on the other. Both latex agglutination and antibody capture assays showed a sensitivity of 100 per cent and specificity of > 98 per cent as compared to culture and isolation in symptomatic patients. However, among asymptomatic carriers sensitivity of 100 per cent and 87.5 per cent and specificity > 95 per cent were observed for latex agglutination and antibody capture assays respectively. Latex agglutination showed no false negative results and sensitivity was not affected by low β-haemolytic counts in asymptomatic children. The rapid tests described here will help in the detection and thereby the management of GAS infection.

Original languageEnglish (US)
Pages (from-to)200-205
Number of pages6
JournalIndian Journal of Medical Research
Volume105
Issue numberMAY
StatePublished - Jun 19 1997

Keywords

  • Antibody capture assay
  • Group-A Streptococcus pyogenes
  • Latex agglutination test
  • Rapid detection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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