TY - JOUR
T1 - Comparison of microchromatography and electrophoresis with elution for hemoglobin A2 (Hb A2) quantitation
AU - Hamilton, S. R.
AU - Miller, M. E.
AU - Jessop, M.
AU - Charache, S.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1979
Y1 - 1979
N2 - Microcolumns prepared in the authors' laboratory, two commercial microchromatography kits, and electrophoresis with elution were compared for Hb A(2) quantitation. Day-to-day imprecision of microchromatographic methods was similar (CV 4.7-6.6%) and somewhat less than electrophoresis with elution (CV 8.0-9.1%). Both commercial kits showed variable imprecision in different lots; one lot of Kit B gave erratic results due to resin leakage. From 49 patient specimens, Kit A microcolumns and those of the authors identified the same 14 patients with an elevated percentage of Hb A(2) and showed good correlation (P=0.90), although Kit A showed constant bias toward higher values. Electrophoresis with elution resulted in a false-positive and a false-negative value, did not correlate well with microcolumns (P = 0.78 and 0.76), and showed proportional bias toward lower values for an elevated percentage of Hb A(2). Commercial kits were convenient, relatively quick, and cost-effective. Frozen, stabilized hemolysates performed well for quality control.
AB - Microcolumns prepared in the authors' laboratory, two commercial microchromatography kits, and electrophoresis with elution were compared for Hb A(2) quantitation. Day-to-day imprecision of microchromatographic methods was similar (CV 4.7-6.6%) and somewhat less than electrophoresis with elution (CV 8.0-9.1%). Both commercial kits showed variable imprecision in different lots; one lot of Kit B gave erratic results due to resin leakage. From 49 patient specimens, Kit A microcolumns and those of the authors identified the same 14 patients with an elevated percentage of Hb A(2) and showed good correlation (P=0.90), although Kit A showed constant bias toward higher values. Electrophoresis with elution resulted in a false-positive and a false-negative value, did not correlate well with microcolumns (P = 0.78 and 0.76), and showed proportional bias toward lower values for an elevated percentage of Hb A(2). Commercial kits were convenient, relatively quick, and cost-effective. Frozen, stabilized hemolysates performed well for quality control.
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U2 - 10.1093/ajcp/71.4.388
DO - 10.1093/ajcp/71.4.388
M3 - Article
C2 - 443196
AN - SCOPUS:0018748219
SN - 0002-9173
VL - 71
SP - 388
EP - 396
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 4
ER -