Comparison of microchromatography and electrophoresis with elution for hemoglobin A2 (Hb A2) quantitation

S. R. Hamilton, M. E. Miller, M. Jessop, S. Charache

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Microcolumns prepared in the authors' laboratory, two commercial microchromatography kits, and electrophoresis with elution were compared for Hb A(2) quantitation. Day-to-day imprecision of microchromatographic methods was similar (CV 4.7-6.6%) and somewhat less than electrophoresis with elution (CV 8.0-9.1%). Both commercial kits showed variable imprecision in different lots; one lot of Kit B gave erratic results due to resin leakage. From 49 patient specimens, Kit A microcolumns and those of the authors identified the same 14 patients with an elevated percentage of Hb A(2) and showed good correlation (P=0.90), although Kit A showed constant bias toward higher values. Electrophoresis with elution resulted in a false-positive and a false-negative value, did not correlate well with microcolumns (P = 0.78 and 0.76), and showed proportional bias toward lower values for an elevated percentage of Hb A(2). Commercial kits were convenient, relatively quick, and cost-effective. Frozen, stabilized hemolysates performed well for quality control.

Original languageEnglish (US)
Pages (from-to)388-396
Number of pages9
JournalAmerican journal of clinical pathology
Volume71
Issue number4
DOIs
StatePublished - 1979

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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