Compatibility of CO 2 laser surgery and fluorescence detection in head and neck cancer cells

Julia Odenthal, Peter Friedl, Robert P. Takes

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Surgical treatment of cancer requires tumor excision with emphasis on function preservation which is achieved in (early stage) laryngeal cancer by transoral carbon dioxide (CO 2 ) laser surgery. Whereas conventional laser surgery is restricted by the surgeon's visual recognition of tumor tissue, new approaches based on fluorescence-guided surgery (FGS) improve the detection of the tumor and its margin. However, it is unclear whether fluorophores are compatible with high-power laser application or whether precision is compromised by laser-induced bleaching of the dye. Methods: We applied topology-controlled 3D laser resection of fluorescent tumors cell in vitro and laser-induced autofluorescence analysis ex vivo. Results: Laser-induced bleaching of fluorescent dyes in the visible and near-infrared light spectrum (650-900 nm) ranges below the resolution range of operation microscopes. Furthermore, specific fluorescent signals in an FGS mouse model is 10 4 higher than laser-induced autofluorescence in mouse tissue. Conclusion: Laser-induced lateral photobleaching is negligible indicating a path forward for fluorescence-guided laser surgery in head and neck cancer.

Original languageEnglish (US)
Pages (from-to)1253-1259
Number of pages7
JournalHead and Neck
Volume41
Issue number5
DOIs
StatePublished - May 2019

Keywords

  • CO laser surgery
  • autofluorescence
  • fluorescence-guided
  • head and neck cancer
  • near-infrared

ASJC Scopus subject areas

  • Otorhinolaryngology

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