Abstract
Background: Surgical treatment of cancer requires tumor excision with emphasis on function preservation which is achieved in (early stage) laryngeal cancer by transoral carbon dioxide (CO 2 ) laser surgery. Whereas conventional laser surgery is restricted by the surgeon's visual recognition of tumor tissue, new approaches based on fluorescence-guided surgery (FGS) improve the detection of the tumor and its margin. However, it is unclear whether fluorophores are compatible with high-power laser application or whether precision is compromised by laser-induced bleaching of the dye. Methods: We applied topology-controlled 3D laser resection of fluorescent tumors cell in vitro and laser-induced autofluorescence analysis ex vivo. Results: Laser-induced bleaching of fluorescent dyes in the visible and near-infrared light spectrum (650-900 nm) ranges below the resolution range of operation microscopes. Furthermore, specific fluorescent signals in an FGS mouse model is 10 4 higher than laser-induced autofluorescence in mouse tissue. Conclusion: Laser-induced lateral photobleaching is negligible indicating a path forward for fluorescence-guided laser surgery in head and neck cancer.
Original language | English (US) |
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Pages (from-to) | 1253-1259 |
Number of pages | 7 |
Journal | Head and Neck |
Volume | 41 |
Issue number | 5 |
DOIs | |
State | Published - May 2019 |
Keywords
- CO laser surgery
- autofluorescence
- fluorescence-guided
- head and neck cancer
- near-infrared
ASJC Scopus subject areas
- Otorhinolaryngology