Computational analysis of light scattering from collagen fiber networks

Dizem Arifler, Ina Pavlova, Ann Gillenwater, Rebecca Richards-Kortum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Neoplastic progression in epithelial tissues is accompanied by structural and morphological changes in the stromal collagen matrix. We used the Finite-Difference Time-Domain (FDTD) method, a popular computational technique for full-vector solution of complex problems in electromagnetics, to establish a relationship between structural properties of collagen fiber networks and light scattering, and to analyze how neoplastic changes alter stromal scattering properties. To create realistic collagen network models, we acquired optical sections from the stroma of fresh normal and neoplastic oral cavity biopsies using fluorescence confocal microscopy. These optical sections were then processed to construct three-dimensional collagen networks of different sizes as FDTD model input. Image analysis revealed that volume fraction of collagen fibers in the stroma decreases with neoplastic progression, and statistical texture features computed suggest that fibers tend to be more disconnected in neoplastic stroma. The FDTD modeling results showed that neoplastic fiber networks have smaller scattering cross-sections compared to normal networks of the same size, whereas high-angle scattering probabilities tend to be higher for neoplastic networks. Characterization of stromal scattering is expected to provide a basis to better interpret spectroscopic optical signals and to develop more reliable computational models to describe photon propagation in epithelial tissues.

Original languageEnglish (US)
Title of host publicationDiagnostic Optical Spectroscopy in Biomedicine IV
PublisherSPIE
ISBN (Print)0819467723, 9780819467720
DOIs
StatePublished - 2007
EventDiagnostic Optical Spectroscopy in Biomedicine IV - Munich, Germany
Duration: Jun 19 2007Jun 21 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6628
ISSN (Print)1605-7422

Other

OtherDiagnostic Optical Spectroscopy in Biomedicine IV
Country/TerritoryGermany
CityMunich
Period6/19/076/21/07

Keywords

  • Collagen matrix
  • Finite-difference time-domain modeling
  • Fluorescence confocal microscopy
  • Light scattering
  • Oral neoplasia

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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