Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine

Rama Rao Amara; Francois Villinger; John D. Altman; Shari L. Lydy; Shawn P. O'Neil; Silvija I. Staprans; David C. Montefiori; Yan Xu; James G. Herndon; Linda S. Wyatt; Maria Angelito Candido; Natalia L. Kozyr; Patricia L. Earl; James M. Smith; Hak Ling Ma; Bennett D. Grimm; Michael L. Hulsey; Harold M. McClure; Janet M. McNicholl; Bernard Moss; Harriet L. Robinson

doi:10.1016/S0264-410X(02)00076-2

Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine

Rama Rao Amara
, Francois Villinger
, John D. Altman
, Shari L. Lydy
, Shawn P. O'Neil
, Silvija I. Staprans
, David C. Montefiori
, Yan Xu
, James G. Herndon
, Linda S. Wyatt
, Maria Angelito Candido
, Natalia L. Kozyr
, Patricia L. Earl
, James M. Smith
, Hak Ling Ma
, Bennett D. Grimm
, Michael L. Hulsey
, Harold M. McClure
, Janet M. McNicholl
, Bernard Moss

Harriet L. Robinson

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here, we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster has controlled a highly pathogenic immunodeficiency virus challenge in a Rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These highly promising findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the AIDS epidemic.

Original language	English (US)
Pages (from-to)	1949-1955
Number of pages	7
Journal	Vaccine
Volume	20
Issue number	15
DOIs	https://doi.org/10.1016/S0264-410X(02)00076-2
State	Published - May 6 2002
Externally published	Yes

Keywords

Boost
Heterologous prime
Mucosal challenge
Multiprotein

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0264-410X(02)00076-2

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Amara, R. R., Villinger, F., Altman, J. D., Lydy, S. L., O'Neil, S. P., Staprans, S. I., Montefiori, D. C., Xu, Y., Herndon, J. G., Wyatt, L. S., Candido, M. A., Kozyr, N. L., Earl, P. L., Smith, J. M., Ma, H. L., Grimm, B. D., Hulsey, M. L., McClure, H. M., McNicholl, J. M., ... Robinson, H. L. (2002). Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine. Vaccine, 20(15), 1949-1955. https://doi.org/10.1016/S0264-410X(02)00076-2

Amara, RR, Villinger, F, Altman, JD, Lydy, SL, O'Neil, SP, Staprans, SI, Montefiori, DC, Xu, Y, Herndon, JG, Wyatt, LS, Candido, MA, Kozyr, NL, Earl, PL, Smith, JM, Ma, HL, Grimm, BD, Hulsey, ML, McClure, HM, McNicholl, JM, Moss, B & Robinson, HL 2002, 'Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine', Vaccine, vol. 20, no. 15, pp. 1949-1955. https://doi.org/10.1016/S0264-410X(02)00076-2

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title = "Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine",

abstract = "Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here, we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster has controlled a highly pathogenic immunodeficiency virus challenge in a Rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These highly promising findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the AIDS epidemic.",

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author = "Amara, \{Rama Rao\} and Francois Villinger and Altman, \{John D.\} and Lydy, \{Shari L.\} and O'Neil, \{Shawn P.\} and Staprans, \{Silvija I.\} and Montefiori, \{David C.\} and Yan Xu and Herndon, \{James G.\} and Wyatt, \{Linda S.\} and Candido, \{Maria Angelito\} and Kozyr, \{Natalia L.\} and Earl, \{Patricia L.\} and Smith, \{James M.\} and Ma, \{Hak Ling\} and Grimm, \{Bennett D.\} and Hulsey, \{Michael L.\} and McClure, \{Harold M.\} and McNicholl, \{Janet M.\} and Bernard Moss and Robinson, \{Harriet L.\}",

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doi = "10.1016/S0264-410X(02)00076-2",

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AU - Amara, Rama Rao

AU - Villinger, Francois

AU - Altman, John D.

AU - Lydy, Shari L.

AU - O'Neil, Shawn P.

AU - Staprans, Silvija I.

AU - Montefiori, David C.

AU - Xu, Yan

AU - Herndon, James G.

AU - Wyatt, Linda S.

AU - Candido, Maria Angelito

AU - Kozyr, Natalia L.

AU - Earl, Patricia L.

AU - Smith, James M.

AU - Ma, Hak Ling

AU - Grimm, Bennett D.

AU - Hulsey, Michael L.

AU - McClure, Harold M.

AU - McNicholl, Janet M.

AU - Moss, Bernard

AU - Robinson, Harriet L.

PY - 2002/5/6

Y1 - 2002/5/6

N2 - Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here, we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster has controlled a highly pathogenic immunodeficiency virus challenge in a Rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These highly promising findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the AIDS epidemic.

AB - Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here, we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster has controlled a highly pathogenic immunodeficiency virus challenge in a Rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These highly promising findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the AIDS epidemic.

KW - Boost

KW - Heterologous prime

KW - Mucosal challenge

KW - Multiprotein

UR - https://www.scopus.com/pages/publications/0037029862

UR - https://www.scopus.com/pages/publications/0037029862#tab=citedBy

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M3 - Article

C2 - 11983252

AN - SCOPUS:0037029862

SN - 0264-410X

VL - 20

SP - 1949

EP - 1955

JO - Vaccine

JF - Vaccine

IS - 15

ER -

Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine

Abstract

Keywords

ASJC Scopus subject areas

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