Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone

Tetsu Kamitani; Hui Ming Chang; Carl Rollins; Gerald L. Waneck; Edward T.H. Yeh

doi:10.1016/s0021-9258(19)36842-5

Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk^- cells by a human cDNA clone

Tetsu Kamitani
, Hui Ming Chang
, Carl Rollins
, Gerald L. Waneck
, Edward T.H. Yeh

Cardiology

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Previous attempts to express glycosylphosphatidyl-inositol-anchored proteins in Ltk^- cells have not been successful because Ltk^- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk^- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.

Original language	English (US)
Pages (from-to)	20733-20736
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	268
Issue number	28
DOIs	https://doi.org/10.1016/s0021-9258(19)36842-5
State	Published - Oct 5 1993

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone",

abstract = "Previous attempts to express glycosylphosphatidyl-inositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.",

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year = "1993",

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T1 - Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone

AU - Kamitani, Tetsu

AU - Chang, Hui Ming

AU - Rollins, Carl

AU - Waneck, Gerald L.

AU - Yeh, Edward T.H.

PY - 1993/10/5

Y1 - 1993/10/5

N2 - Previous attempts to express glycosylphosphatidyl-inositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.

AB - Previous attempts to express glycosylphosphatidyl-inositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.

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Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk^- cells by a human cDNA clone

Abstract

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