TY - JOUR
T1 - Correlation of in-vivo imaging with histopathology
T2 - A review
AU - Sen, Anando
AU - Troncoso, Patricia
AU - Venkatesan, Aradhana
AU - Pagel, Mark D.
AU - Nijkamp, Jasper A.
AU - He, Yulun
AU - Lesage, Anne Cecille
AU - Woodland, McKell
AU - Brock, Kristy K.
N1 - Funding Information:
This research was supported in part by resources of the Image Guided Cancer Therapy Research Program at the University of Texas M D Anderson Cancer Center, the Helen Black Image-Guided Fund and the Tumor Measurement Initiative. Editorial support was provided by Bryan Tutt, Scientific Editor, Research Medical Library at M D Anderson Cancer Center. The authors thank Kelly Kage, MFA, CMI for her assistance with the graphics in this article.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/11
Y1 - 2021/11
N2 - Despite tremendous advancements in in vivo imaging modalities, there remains substantial uncertainty with respect to tumor delineation on in these images. Histopathology remains the gold standard for determining the extent of malignancy, with in vivo imaging to histopathologic correlation enabling spatial comparisons. In this review, the steps necessary for successful imaging to histopathologic correlation are described, including in vivo imaging, resection, fixation, specimen sectioning (sectioning technique, securing technique, orientation matching, slice matching), microtome sectioning and staining, correlation (including image registration) and performance evaluation. The techniques used for each of these steps are also discussed. Hundreds of publications from the past 20 years were surveyed, and 62 selected for detailed analysis. For these 62 publications, each stage of the correlative pathology process (and the sub-steps of specimen sectioning) are listed. A statistical analysis was conducted based on 19 studies that reported target registration error as their performance metric. While some methods promise greater accuracy, they may be expensive. Due to the complexity of the processes involved, correlative pathology studies generally include a small number of subjects, which hinders advanced developments in this field.
AB - Despite tremendous advancements in in vivo imaging modalities, there remains substantial uncertainty with respect to tumor delineation on in these images. Histopathology remains the gold standard for determining the extent of malignancy, with in vivo imaging to histopathologic correlation enabling spatial comparisons. In this review, the steps necessary for successful imaging to histopathologic correlation are described, including in vivo imaging, resection, fixation, specimen sectioning (sectioning technique, securing technique, orientation matching, slice matching), microtome sectioning and staining, correlation (including image registration) and performance evaluation. The techniques used for each of these steps are also discussed. Hundreds of publications from the past 20 years were surveyed, and 62 selected for detailed analysis. For these 62 publications, each stage of the correlative pathology process (and the sub-steps of specimen sectioning) are listed. A statistical analysis was conducted based on 19 studies that reported target registration error as their performance metric. While some methods promise greater accuracy, they may be expensive. Due to the complexity of the processes involved, correlative pathology studies generally include a small number of subjects, which hinders advanced developments in this field.
KW - Correlative pathology
KW - Image registration
KW - Imaging-pathology correlation
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U2 - 10.1016/j.ejrad.2021.109964
DO - 10.1016/j.ejrad.2021.109964
M3 - Review article
C2 - 34619617
AN - SCOPUS:85116412449
SN - 0720-048X
VL - 144
JO - European Journal of Radiology
JF - European Journal of Radiology
M1 - 109964
ER -