Correlation of Notch1 expression and activation to cisplatin-sensitivity of head and neck squamous cell carcinoma

Zu Ping Zhang, Yu Lan Sun, Li Fu, Feng Gu, Lun Zhang, Xi Shan Hao

    Research output: Contribution to journalArticle

    19 Scopus citations

    Abstract

    Background and Objective: Notch1 belongs to the Notch family of transmembrane receptors and plays an important role in tumor cell proliferation and apoptosis. Notch1 affects chemosensitivity of tumors. However, its correlation to cisplatin (DDP)-sensitivity of head and neck squamous cell carcinoma is unclear. This study was to identify the expression of Notch1 in head and neck squamous cell carcinoma, and investigate its influence on the DDP-sensitivity. Methods: Twenty-five fresh specimens of head and neck squamous cell carcinoma were subjected to primary cell culture. DDP-sensitivity of tumor cells was detected using collagen gel droplet embedded culture-drug sensitivity test (CD-DST). The expression of Notch1 in head and neck squamous cell carcinoma, normal squamous epithelium, and tongue squamous cell carcinoma Tb3.1 cells was detected by immunohistochemistry or immunocytochemistry. Tb3.1 cells were divided into four groups, and received treatment of DMSO, DAPT, DMSO plus DDP, DAPT plus DDP, respectively. The absorbance of the four groups was detected by CD-DST to evaluate DDP-sensitivity. Results: The positive rate of Notch1 was significantly higher in head and neck squamous cell carcinoma than in normal squamous epithelium (100% vs. 35%, p < 0.001), and it was negatively correlated to DDP-sensitivity (r = -0.705, p < 0.01). There was no difference in absorbance between DMSO group and DAPT group (155.4 ± 2.3 vs. 154.7 ± 1.2, p > 0.05), while the absorbance was significantly higher in DMSO plus DDP group than in DAPT plus DDP (33.9 ± 1.3 vs. 26.6 ± 1.1, p < 0.05). Conclusions: Notch1 expression is negatively correlated to DDP-sensitivity of head and neck squamous cell carcinoma, and could be used to predict DDP-sensitivity. DAPT can enhance DDP-sensitivity of Tb3.1 cells via blocking Notch1 signaling.

    Original languageEnglish (US)
    Pages (from-to)100-103
    Number of pages4
    JournalChinese journal of cancer
    Volume28
    Issue number2
    StatePublished - Oct 5 2009

      Fingerprint

    Keywords

    • Chemosensitivity
    • Cisplatin
    • Head and neck neoplasm
    • Notch 1
    • Squamous cell carcinoma

    ASJC Scopus subject areas

    • Oncology

    Cite this

    Zhang, Z. P., Sun, Y. L., Fu, L., Gu, F., Zhang, L., & Hao, X. S. (2009). Correlation of Notch1 expression and activation to cisplatin-sensitivity of head and neck squamous cell carcinoma. Chinese journal of cancer, 28(2), 100-103.