Correlations between FGFR2 gene polymorphism and susceptibility of breast cancer

Hong Meng Zhao, Bin Zhang, Hui Yao, Xiao Li Li, Yan Qun Song, Xin Wang, Xiao Xia Liu, Xuchen Cao

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    Abstract

    Objective: To investigate the relationship between the fibroblast growth factor 2 (FGFR2) gene polymorphism rs2981582 and the susceptibility of breast cancer in Chinese women. Methods: A single nucleotide polymorphism (SNP) of rs2981582 loci in FGFR2 gene (C/T) was detected using Tm-shifting real-time fluorescent quantitative PCR. DNA in the specimen was labeled by the fluorescent dye SYBR Green I. By adding tails of different lengths in the 5′-end of specific primers, the peaks of different genotypes in the solubility curve could be varied. The criterion was defined as follows: a Tm lower than or equivalent to 84.6°C meant a homozygote CC, a Tm higher than or equivalent to 87.5°C meant a homozygote TT, and a Tm between the two thresholds meant a heterozygote TC. Using this method, 936 patients with breast cancer and 471 patients with benign breast diseases were tested to analyze the relationship between this SNP and carcinogenesis of the breast cancer. Results: The frequencies of the genotypes CC, CT, and TT were respectively 426 (44.56%), 400 (41.84%) and 130 (13.60%) in the breast cancer group and 234 (49.68%), 181 (38.43%) and 56 (11.89%) in the control group. There was no statistical significance in the differences between the two groups (P=0.183). Further stratified analysis demonstrated that the frequencies of the genotypes CC, CT and TT were respectively 189 (41.27%), 202 (46.12%) and 67 (14.63%) in the positive ER subgroup of breast cancer, with a significant difference when compared with the control group (P=0.035). However, the frequencies of the three genotypes were 237 (47.59%), 198 (39.75%) and 63 (12.65%), respectively, in the negative ER subgroup of breast cancer, without a significant difference when compared with the control group (P=0.802). Conclusion: There is a significant correlation between the SNP rs2981582 of intron 2 in FG-FR2 gene and ER-positive breast cancer. Our paper also demonstrates this new detection method is suitable for SNP typing a large number of samples because it is cheap, easy and simple to use. Keywords fluorescent quantitative PCR, single nucleotide polymorphisms, fibroblast growth factor receptor 2, breast cancer.

    Original languageEnglish (US)
    Pages (from-to)626-629
    Number of pages4
    JournalChinese Journal of Clinical Oncology
    Volume37
    Issue number11
    DOIs
    StatePublished - Jun 15 2010

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    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

    Cite this

    Zhao, H. M., Zhang, B., Yao, H., Li, X. L., Song, Y. Q., Wang, X., Liu, X. X., & Cao, X. (2010). Correlations between FGFR2 gene polymorphism and susceptibility of breast cancer. Chinese Journal of Clinical Oncology, 37(11), 626-629. https://doi.org/10.3969/j.issn.1000-8179.2010.11.008