The authors apologize for the error in Fig. 2E- The Figure legend should have made it clear that M0 macrophages: the top panel was a low magnification and the mid panel was a higher magnification of the same cell. There was no intent of duplication. Fig. 2 corrected legend. Fig. 2 Macrophage and exosome characterization. (A) Morphology of undifferentiated monocytes at day 0, showing a cell diameter of <30 μm under 3D atomic force microscopy (AFM). (B) Morphology of M0 macrophages after 3 days of treatment, showing a cell diameter of >30 μm under 3D AFM. (C) Morphology of M2 macrophages after 24 h of treatment with IL-4 and IL-13, showing a cell diameter of >100 μm under 3D AFM. (D, E) Characteristic morphology of M2 macrophages compared with M0 macrophages: cell elongation to modulate macrophage phenotype polarization (AFM and 3D optical microscopy); E) M0 macrophages: top panel- low magnification and mid panel- higher magnification of the same cell (F) Immunoblotting to validate the macrophage markers CD206, CD68, and CD163 in differentiated THP-1 cells and undifferentiated THP-1 cells. (G) Flow cytometry analysis to verify differential expression of M2 markers CD163 and CD206 between differentiated and undifferentiated THP-1 cells (P < .05). (H) Quantification of isolated exosomes from tumor-associated macrophages (Nano sight).
ASJC Scopus subject areas
- Cell Biology