CORRIGENDUM: GATA3 as a master regulator for interactions of tumor-associated macrophages with high-grade serous ovarian carcinoma (Cellular Signalling (2020) 68, (S0898656820300164), (10.1016/j.cellsig.2020.109539))

Amr Ahmed El-Arabey, Merve Denizli, Pinar Kanlikilicer, Recep Bayraktar, Cristina Ivan, Mohammed Rashed, Nashwa Kabil, Bulent Ozpolat, George A. Calin, Salama Abdou Salama, Adel Rashad Abd-Allah, Anil K. Sood, Gabriel Lopez-Berestein

Research output: Contribution to journalComment/debatepeer-review

Abstract

The authors apologize for the error in Fig. 2E- The Figure legend should have made it clear that M0 macrophages: the top panel was a low magnification and the mid panel was a higher magnification of the same cell. There was no intent of duplication. Fig. 2 corrected legend. Fig. 2 Macrophage and exosome characterization. (A) Morphology of undifferentiated monocytes at day 0, showing a cell diameter of <30 μm under 3D atomic force microscopy (AFM). (B) Morphology of M0 macrophages after 3 days of treatment, showing a cell diameter of >30 μm under 3D AFM. (C) Morphology of M2 macrophages after 24 h of treatment with IL-4 and IL-13, showing a cell diameter of >100 μm under 3D AFM. (D, E) Characteristic morphology of M2 macrophages compared with M0 macrophages: cell elongation to modulate macrophage phenotype polarization (AFM and 3D optical microscopy); E) M0 macrophages: top panel- low magnification and mid panel- higher magnification of the same cell (F) Immunoblotting to validate the macrophage markers CD206, CD68, and CD163 in differentiated THP-1 cells and undifferentiated THP-1 cells. (G) Flow cytometry analysis to verify differential expression of M2 markers CD163 and CD206 between differentiated and undifferentiated THP-1 cells (P < .05). (H) Quantification of isolated exosomes from tumor-associated macrophages (Nano sight).

Original languageEnglish (US)
Article number110147
JournalCellular Signalling
Volume89
DOIs
StatePublished - Jan 2022

ASJC Scopus subject areas

  • Cell Biology

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