COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers

Rayka Yokoo, Karl A. Zawadzki, Kentaro Nabeshima, Melanie Drake, Swathi Arur, Anne M. Villeneuve

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Crossovers (COs) between homologous chromosomes ensure their faithful segregation during meiosis. We identify C. elegans COSA-1, a cyclin-related protein conserved in metazoa, as a key component required to convert meiotic double-strand breaks (DSBs) into COs. During late meiotic prophase, COSA-1 localizes to foci that correspond to the single CO site on each homolog pair and indicate sites of eventual concentration of other conserved CO proteins. Chromosomes gain and lose competence to load CO proteins during meiotic progression, with competence to load COSA-1 requiring prior licensing. Our data further suggest a self-reinforcing mechanism maintaining CO designation. Modeling of a nonlinear dose-response relationship between IR-induced DSBs and COSA-1 foci reveals efficient conversion of DSBs into COs when DSBs are limiting and a robust capacity to limit cytologically differentiated CO sites when DSBs are in excess. COSA-1 foci serve as a unique live cell readout for investigating CO formation and CO interference.

Original languageEnglish (US)
Pages (from-to)75-87
Number of pages13
JournalCell
Volume149
Issue number1
DOIs
StatePublished - Mar 30 2012

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Licensure
Reinforcement
Homeostasis
Chromosomes
Mental Competency
Meiotic Prophase I
Proteins
Cyclins
Meiosis
Reinforcement (Psychology)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers. / Yokoo, Rayka; Zawadzki, Karl A.; Nabeshima, Kentaro; Drake, Melanie; Arur, Swathi; Villeneuve, Anne M.

In: Cell, Vol. 149, No. 1, 30.03.2012, p. 75-87.

Research output: Contribution to journalArticle

Yokoo, Rayka ; Zawadzki, Karl A. ; Nabeshima, Kentaro ; Drake, Melanie ; Arur, Swathi ; Villeneuve, Anne M. / COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers. In: Cell. 2012 ; Vol. 149, No. 1. pp. 75-87.
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