Crude extracts of Solanum lyratum induced cytotoxicity and apoptosis in a human colon adenocarcinoma cell line (colo 205)

Shu Chun Hsu, Jih Hung Lu, Chao Lin Kuo, Jai Sing Yang, Meng Wei Lin, Guang Wei Chen, Chin Cheng Su, Hsu Fung Lu, Jing Gung Chung

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effects of the crude extract of Solanum lyratum (SLE) on human colon cancer colo 205 cells were investigated. The cell viability, morphological changes of the cells, cell cycle arrest, apoptosis, reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm) and cell cycle- and apoptosis-associated protein levels and gene expressions were examined in colo 205 cells after exposure to various concentrations of SLE for different time periods. The results indicated that SLE decreased the percentage of viable colo 205 cells accompanied by morphological changes. The most effective concentration of SLE was 300 μg/ml (SLE 300) and this concentration was used for further investigations. SLE induced S-phase arrest and apoptosis (sub-G1) in the colo 205 cells and those effects were dose- and time-dependent. DAPI staining and DNA gel electrophoresis confirmed that SLE induced apoptosis in colo 205 cells. Flow cytometric analysis also showed that SLE 300 promoted ROS production and decreased the ΔΨm. Western blotting analysis indicated that SLE 300 increased Bax levels and decreased Bcl-2 levels, which caused the loss of ΔΨm followed by cytochrome c release and caspase-9 and -3 activation, finally leading to apoptosis. SLE 300 also promoted p53 and p27, but decreased the levels of cyclin B1 thus causing S-phase arrest. The gene expression associated with those proteins was also confirmed by PCR methods. The findings show that SLE might be used as a colon cancer therapeutic agent in the future.

Original languageEnglish (US)
Pages (from-to)1045-1054
Number of pages10
JournalAnticancer research
Volume28
Issue number2 A
StatePublished - Mar 1 2008

Fingerprint

Solanum
Complex Mixtures
Colon
Adenocarcinoma
Apoptosis
Cell Line
S Phase
Colonic Neoplasms
Reactive Oxygen Species
Cyclin B1
Gene Expression
Caspase 9
Mitochondrial Membrane Potential
Cell Cycle Checkpoints
Cytochromes c
Caspase 3
Electrophoresis
Cell Survival
Cell Cycle
Proteins

Keywords

  • Apoptosis
  • Colon adenocarcinoma cell lines
  • Cytotoxicity
  • Solanum lyratum extracts

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Crude extracts of Solanum lyratum induced cytotoxicity and apoptosis in a human colon adenocarcinoma cell line (colo 205). / Hsu, Shu Chun; Lu, Jih Hung; Kuo, Chao Lin; Yang, Jai Sing; Lin, Meng Wei; Chen, Guang Wei; Su, Chin Cheng; Lu, Hsu Fung; Chung, Jing Gung.

In: Anticancer research, Vol. 28, No. 2 A, 01.03.2008, p. 1045-1054.

Research output: Contribution to journalArticle

Hsu, SC, Lu, JH, Kuo, CL, Yang, JS, Lin, MW, Chen, GW, Su, CC, Lu, HF & Chung, JG 2008, 'Crude extracts of Solanum lyratum induced cytotoxicity and apoptosis in a human colon adenocarcinoma cell line (colo 205)', Anticancer research, vol. 28, no. 2 A, pp. 1045-1054.
Hsu, Shu Chun ; Lu, Jih Hung ; Kuo, Chao Lin ; Yang, Jai Sing ; Lin, Meng Wei ; Chen, Guang Wei ; Su, Chin Cheng ; Lu, Hsu Fung ; Chung, Jing Gung. / Crude extracts of Solanum lyratum induced cytotoxicity and apoptosis in a human colon adenocarcinoma cell line (colo 205). In: Anticancer research. 2008 ; Vol. 28, No. 2 A. pp. 1045-1054.
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AU - Lin, Meng Wei

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AU - Su, Chin Cheng

AU - Lu, Hsu Fung

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AB - The effects of the crude extract of Solanum lyratum (SLE) on human colon cancer colo 205 cells were investigated. The cell viability, morphological changes of the cells, cell cycle arrest, apoptosis, reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm) and cell cycle- and apoptosis-associated protein levels and gene expressions were examined in colo 205 cells after exposure to various concentrations of SLE for different time periods. The results indicated that SLE decreased the percentage of viable colo 205 cells accompanied by morphological changes. The most effective concentration of SLE was 300 μg/ml (SLE 300) and this concentration was used for further investigations. SLE induced S-phase arrest and apoptosis (sub-G1) in the colo 205 cells and those effects were dose- and time-dependent. DAPI staining and DNA gel electrophoresis confirmed that SLE induced apoptosis in colo 205 cells. Flow cytometric analysis also showed that SLE 300 promoted ROS production and decreased the ΔΨm. Western blotting analysis indicated that SLE 300 increased Bax levels and decreased Bcl-2 levels, which caused the loss of ΔΨm followed by cytochrome c release and caspase-9 and -3 activation, finally leading to apoptosis. SLE 300 also promoted p53 and p27, but decreased the levels of cyclin B1 thus causing S-phase arrest. The gene expression associated with those proteins was also confirmed by PCR methods. The findings show that SLE might be used as a colon cancer therapeutic agent in the future.

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