TY - JOUR
T1 - Cyclin D3 interacts with vitamin D receptor and regulates its transcription activity
AU - Jian, Yongzhi
AU - Yan, Jun
AU - Wang, Hanzhou
AU - Chen, Chen
AU - Sun, Maoyun
AU - Jiang, Jianhai
AU - Lu, Jieqiong
AU - Yang, Yanzhong
AU - Gu, Jianxin
N1 - Funding Information:
This work was supported by National Natural Scientific Foundation of China (30470442 and 30330320) and CNHLPP (2004BA711A19) and a Grant from the Development of Science and Technology of Shanghai (02DJ14002). We thank Drs. Paul N. MacDonald, Z.H. Yuan, C.J. Sherr, and Jane B. Lian for the plasmids described in Experimental procedures.
PY - 2005/9/30
Y1 - 2005/9/30
N2 - D-type cyclins are essential for the progression through the G1 phase of the cell cycle. Besides serving as cell cycle regulators, D-type cyclins were recently reported to have transcription regulation functions. Here, we report that cyclin D3 is a new interacting partner of vitamin D receptor (VDR), a member of the superfamily of nuclear receptors for steroid hormones, thyroid hormone, and the fat-soluble vitamins A and D. The interaction was confirmed with methods of yeast two-hybrid system, in vitro binding analysis and in vivo co-immunoprecipitation. Cyclin D3 interacted with VDR in a ligand-independent manner, but treatment of the ligand, 1,25-dihydroxyvitamin D3, strengthened the interaction. Confocal microscopy analysis showed that ligand-activated VDR led to an accumulation of cyclin D3 in the nuclear region. Cyclin D3 up-regulated transcriptional activity of VDR and this effect was counteracted by overexpression of CDK4 and CDK6. These findings provide us a new clue to understand the transcription regulation functions of D-type cyclins.
AB - D-type cyclins are essential for the progression through the G1 phase of the cell cycle. Besides serving as cell cycle regulators, D-type cyclins were recently reported to have transcription regulation functions. Here, we report that cyclin D3 is a new interacting partner of vitamin D receptor (VDR), a member of the superfamily of nuclear receptors for steroid hormones, thyroid hormone, and the fat-soluble vitamins A and D. The interaction was confirmed with methods of yeast two-hybrid system, in vitro binding analysis and in vivo co-immunoprecipitation. Cyclin D3 interacted with VDR in a ligand-independent manner, but treatment of the ligand, 1,25-dihydroxyvitamin D3, strengthened the interaction. Confocal microscopy analysis showed that ligand-activated VDR led to an accumulation of cyclin D3 in the nuclear region. Cyclin D3 up-regulated transcriptional activity of VDR and this effect was counteracted by overexpression of CDK4 and CDK6. These findings provide us a new clue to understand the transcription regulation functions of D-type cyclins.
KW - Cyclin D3
KW - Transcription activity
KW - VDR
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U2 - 10.1016/j.bbrc.2005.07.141
DO - 10.1016/j.bbrc.2005.07.141
M3 - Article
C2 - 16105657
AN - SCOPUS:23944473614
SN - 0006-291X
VL - 335
SP - 739
EP - 748
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 3
ER -