Cyclin is a component of maturation-promoting factor from Xenopus

Jean Gautier; Jeremy Minshull; Manfred Lohka; Michael Glotzer; Tim Hunt; James L. Maller

doi:10.1016/0092-8674(90)90599-A

Cyclin is a component of maturation-promoting factor from Xenopus

Jean Gautier
, Jeremy Minshull
, Manfred Lohka
, Michael Glotzer
, Tim Hunt
, James L. Maller

Research output: Contribution to journal › Article › peer-review

583 Scopus citations

Abstract

Highly purified maturation-promoting factor (MPF) from Xenopus eggs contains both cyclin B1 and cyclin B2 as shown by Western blotting and immunoprecipitation using Xenopus anti-B-type cyclin antibodies. Immunoprecipitates with these antibodies display the histone H1 kinase activity characteristic of MPF, for which exogenously added B1 and B2 cyclins are both substrates. Protein kinase activity against cyclin oscillates in maturing oocytes and activated eggs with the same kinetics as p34^cdc2 kinase activity. These data indicate that B-type cyclin is the other component of MPF besides p34^cdc2.

Original language	English (US)
Pages (from-to)	487-494
Number of pages	8
Journal	Cell
Volume	60
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(90)90599-A
State	Published - Feb 9 1990
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(90)90599-A

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title = "Cyclin is a component of maturation-promoting factor from Xenopus",

abstract = "Highly purified maturation-promoting factor (MPF) from Xenopus eggs contains both cyclin B1 and cyclin B2 as shown by Western blotting and immunoprecipitation using Xenopus anti-B-type cyclin antibodies. Immunoprecipitates with these antibodies display the histone H1 kinase activity characteristic of MPF, for which exogenously added B1 and B2 cyclins are both substrates. Protein kinase activity against cyclin oscillates in maturing oocytes and activated eggs with the same kinetics as p34cdc2 kinase activity. These data indicate that B-type cyclin is the other component of MPF besides p34cdc2.",

author = "Jean Gautier and Jeremy Minshull and Manfred Lohka and Michael Glotzer and Tim Hunt and Maller, \{James L.\}",

year = "1990",

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T1 - Cyclin is a component of maturation-promoting factor from Xenopus

AU - Gautier, Jean

AU - Minshull, Jeremy

AU - Lohka, Manfred

AU - Glotzer, Michael

AU - Hunt, Tim

AU - Maller, James L.

PY - 1990/2/9

Y1 - 1990/2/9

N2 - Highly purified maturation-promoting factor (MPF) from Xenopus eggs contains both cyclin B1 and cyclin B2 as shown by Western blotting and immunoprecipitation using Xenopus anti-B-type cyclin antibodies. Immunoprecipitates with these antibodies display the histone H1 kinase activity characteristic of MPF, for which exogenously added B1 and B2 cyclins are both substrates. Protein kinase activity against cyclin oscillates in maturing oocytes and activated eggs with the same kinetics as p34cdc2 kinase activity. These data indicate that B-type cyclin is the other component of MPF besides p34cdc2.

AB - Highly purified maturation-promoting factor (MPF) from Xenopus eggs contains both cyclin B1 and cyclin B2 as shown by Western blotting and immunoprecipitation using Xenopus anti-B-type cyclin antibodies. Immunoprecipitates with these antibodies display the histone H1 kinase activity characteristic of MPF, for which exogenously added B1 and B2 cyclins are both substrates. Protein kinase activity against cyclin oscillates in maturing oocytes and activated eggs with the same kinetics as p34cdc2 kinase activity. These data indicate that B-type cyclin is the other component of MPF besides p34cdc2.

UR - https://www.scopus.com/pages/publications/0025012866

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DO - 10.1016/0092-8674(90)90599-A

M3 - Article

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SN - 0092-8674

VL - 60

SP - 487

EP - 494

JO - Cell

JF - Cell

IS - 3

ER -

Cyclin is a component of maturation-promoting factor from Xenopus

Abstract

ASJC Scopus subject areas

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