Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection

Xinwen Wang, Scott A. LeMaire, Li Chen, Stacey A. Carter, Ying H. Shen, Yehua Gan, Heather Bartsch, Jonathan A. Wilks, Budi Utama, Hesheng Ou, Robert W. Thompson, Joseph S. Coselli, Li Wang Xing

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Abstract

Background. Thoracic aortic aneurysmal diseases are characterized by degeneration of elastin within the aortic wall. Although proteinases, such as matrix metalloproteinase, appear to contribute to elastin degradation, little is known about the role of elastic fiber assembly in such diseases. Fibulin-5 is an extracellular protein that is expressed in the vascular basement membrane and regulates elastic fiber assembly by microfibril machinery. In this study, we examined whether thoracic aortic dissection (TAD) is associated with abnormal fibulin-5 expression. Methods. Intraoperative aortic samples were obtained from 21 patients with proximal aortic dissection. Control aortic tissue was obtained from 11 organ donors, heart transplant recipients, and patients undergoing coronary artery bypass. An in vitro culture of vascular smooth muscle cells was obtained from 2 TAD patients and 1 control subject. To evaluate elastin expression, we stained tissue sections with Verhoeff-Van Gieson stain. Fibulin-5 messenger RNA (mRNA) expression was determined by quantitative real-time reverse-transcriptase-polymerase chain reaction. Results. Aortic fibulin-5 mRNA and elastin content were decreased in TAD patients, compared with controls (P = .001 and P = .02, respectively). Decreased fibulin-5 expression strongly correlated with decreased amounts and fragmentation of elastin in aortic samples from patients with TAD (r = 0.83, P < .0001 and F = 20.7, P < .0001 respectively). The fibulin-5 mRNA in aortic vascular smooth muscle cells collected from TAD demonstrated a 38% decrease in expression, compared with the control. Conclusions. Patients with proximal aortic dissection exhibited significantly decreased expression of aortic fibulin-5. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly.

Original languageEnglish (US)
Pages (from-to)352-359
Number of pages8
JournalSurgery
Volume138
Issue number2
DOIs
StatePublished - Aug 1 2005

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Elastin
Dissection
Thorax
Elastic Tissue
Vascular Smooth Muscle
Messenger RNA
Smooth Muscle Myocytes
Microfibrils
Aortic Diseases
fibulin
Reverse Transcriptase Polymerase Chain Reaction
Matrix Metalloproteinases
Basement Membrane
Coronary Artery Bypass
Blood Vessels
Real-Time Polymerase Chain Reaction
Peptide Hydrolases
Coloring Agents
Tissue Donors

ASJC Scopus subject areas

  • Surgery

Cite this

Wang, X., LeMaire, S. A., Chen, L., Carter, S. A., Shen, Y. H., Gan, Y., ... Xing, L. W. (2005). Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection. Surgery, 138(2), 352-359. https://doi.org/10.1016/j.surg.2005.06.006

Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection. / Wang, Xinwen; LeMaire, Scott A.; Chen, Li; Carter, Stacey A.; Shen, Ying H.; Gan, Yehua; Bartsch, Heather; Wilks, Jonathan A.; Utama, Budi; Ou, Hesheng; Thompson, Robert W.; Coselli, Joseph S.; Xing, Li Wang.

In: Surgery, Vol. 138, No. 2, 01.08.2005, p. 352-359.

Research output: Contribution to journalArticle

Wang, X, LeMaire, SA, Chen, L, Carter, SA, Shen, YH, Gan, Y, Bartsch, H, Wilks, JA, Utama, B, Ou, H, Thompson, RW, Coselli, JS & Xing, LW 2005, 'Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection', Surgery, vol. 138, no. 2, pp. 352-359. https://doi.org/10.1016/j.surg.2005.06.006
Wang, Xinwen ; LeMaire, Scott A. ; Chen, Li ; Carter, Stacey A. ; Shen, Ying H. ; Gan, Yehua ; Bartsch, Heather ; Wilks, Jonathan A. ; Utama, Budi ; Ou, Hesheng ; Thompson, Robert W. ; Coselli, Joseph S. ; Xing, Li Wang. / Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection. In: Surgery. 2005 ; Vol. 138, No. 2. pp. 352-359.
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abstract = "Background. Thoracic aortic aneurysmal diseases are characterized by degeneration of elastin within the aortic wall. Although proteinases, such as matrix metalloproteinase, appear to contribute to elastin degradation, little is known about the role of elastic fiber assembly in such diseases. Fibulin-5 is an extracellular protein that is expressed in the vascular basement membrane and regulates elastic fiber assembly by microfibril machinery. In this study, we examined whether thoracic aortic dissection (TAD) is associated with abnormal fibulin-5 expression. Methods. Intraoperative aortic samples were obtained from 21 patients with proximal aortic dissection. Control aortic tissue was obtained from 11 organ donors, heart transplant recipients, and patients undergoing coronary artery bypass. An in vitro culture of vascular smooth muscle cells was obtained from 2 TAD patients and 1 control subject. To evaluate elastin expression, we stained tissue sections with Verhoeff-Van Gieson stain. Fibulin-5 messenger RNA (mRNA) expression was determined by quantitative real-time reverse-transcriptase-polymerase chain reaction. Results. Aortic fibulin-5 mRNA and elastin content were decreased in TAD patients, compared with controls (P = .001 and P = .02, respectively). Decreased fibulin-5 expression strongly correlated with decreased amounts and fragmentation of elastin in aortic samples from patients with TAD (r = 0.83, P < .0001 and F = 20.7, P < .0001 respectively). The fibulin-5 mRNA in aortic vascular smooth muscle cells collected from TAD demonstrated a 38{\%} decrease in expression, compared with the control. Conclusions. Patients with proximal aortic dissection exhibited significantly decreased expression of aortic fibulin-5. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly.",
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T1 - Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection

AU - Wang, Xinwen

AU - LeMaire, Scott A.

AU - Chen, Li

AU - Carter, Stacey A.

AU - Shen, Ying H.

AU - Gan, Yehua

AU - Bartsch, Heather

AU - Wilks, Jonathan A.

AU - Utama, Budi

AU - Ou, Hesheng

AU - Thompson, Robert W.

AU - Coselli, Joseph S.

AU - Xing, Li Wang

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Background. Thoracic aortic aneurysmal diseases are characterized by degeneration of elastin within the aortic wall. Although proteinases, such as matrix metalloproteinase, appear to contribute to elastin degradation, little is known about the role of elastic fiber assembly in such diseases. Fibulin-5 is an extracellular protein that is expressed in the vascular basement membrane and regulates elastic fiber assembly by microfibril machinery. In this study, we examined whether thoracic aortic dissection (TAD) is associated with abnormal fibulin-5 expression. Methods. Intraoperative aortic samples were obtained from 21 patients with proximal aortic dissection. Control aortic tissue was obtained from 11 organ donors, heart transplant recipients, and patients undergoing coronary artery bypass. An in vitro culture of vascular smooth muscle cells was obtained from 2 TAD patients and 1 control subject. To evaluate elastin expression, we stained tissue sections with Verhoeff-Van Gieson stain. Fibulin-5 messenger RNA (mRNA) expression was determined by quantitative real-time reverse-transcriptase-polymerase chain reaction. Results. Aortic fibulin-5 mRNA and elastin content were decreased in TAD patients, compared with controls (P = .001 and P = .02, respectively). Decreased fibulin-5 expression strongly correlated with decreased amounts and fragmentation of elastin in aortic samples from patients with TAD (r = 0.83, P < .0001 and F = 20.7, P < .0001 respectively). The fibulin-5 mRNA in aortic vascular smooth muscle cells collected from TAD demonstrated a 38% decrease in expression, compared with the control. Conclusions. Patients with proximal aortic dissection exhibited significantly decreased expression of aortic fibulin-5. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly.

AB - Background. Thoracic aortic aneurysmal diseases are characterized by degeneration of elastin within the aortic wall. Although proteinases, such as matrix metalloproteinase, appear to contribute to elastin degradation, little is known about the role of elastic fiber assembly in such diseases. Fibulin-5 is an extracellular protein that is expressed in the vascular basement membrane and regulates elastic fiber assembly by microfibril machinery. In this study, we examined whether thoracic aortic dissection (TAD) is associated with abnormal fibulin-5 expression. Methods. Intraoperative aortic samples were obtained from 21 patients with proximal aortic dissection. Control aortic tissue was obtained from 11 organ donors, heart transplant recipients, and patients undergoing coronary artery bypass. An in vitro culture of vascular smooth muscle cells was obtained from 2 TAD patients and 1 control subject. To evaluate elastin expression, we stained tissue sections with Verhoeff-Van Gieson stain. Fibulin-5 messenger RNA (mRNA) expression was determined by quantitative real-time reverse-transcriptase-polymerase chain reaction. Results. Aortic fibulin-5 mRNA and elastin content were decreased in TAD patients, compared with controls (P = .001 and P = .02, respectively). Decreased fibulin-5 expression strongly correlated with decreased amounts and fragmentation of elastin in aortic samples from patients with TAD (r = 0.83, P < .0001 and F = 20.7, P < .0001 respectively). The fibulin-5 mRNA in aortic vascular smooth muscle cells collected from TAD demonstrated a 38% decrease in expression, compared with the control. Conclusions. Patients with proximal aortic dissection exhibited significantly decreased expression of aortic fibulin-5. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly.

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U2 - 10.1016/j.surg.2005.06.006

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