Deficient CD4+ CD25+ FOXP3+ T regulatory cells in acquired aplastic anemia

Elena E. Solomou, Katayoun Rezvani, Stephan Mielke, Daniela Malide, Keyvan Keyvanfar, Valeria Visconte, Sachiko Kajigaya, A. John Barrett, Neal S. Young

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Regulatory T cells are believed to control the development and progression of autoimmunity by suppressing autoreactive T cells. Decreased numbers ofCD4+CD25+ FOXP3+ T cells (Tregs) are associated with impaired immune homeostasis and development of autoimmune diseases. The transcription factors FOXP3 and NFAT1 have key roles in regulatory T-cell development and function. We show that Tregs are decreased at presentation in almost all patients with aplastic anemia; FOXP3 protein and mRNA levels also are significantly lower in patients with aplastic anemia and NFAT1 protein levels are decreased or absent. Transfection of FOXP3-deficient CD4 +CD25+ T cells from patients with a plasmid encoding wild-type NFAT1 resulted in increased FOXP3 expression in these cells. By NFAT1 knockdown in CD4+CD25+ T cells, FOXP3 expression was decreased when NFAT1 expression was decreased. Our findings indicate that decreased NFAT1 could explain low FOXP3 expression and diminished Treg frequency in aplastic anemia. Treg defects are now implicated in autoimmune marrow failure.

Original languageEnglish (US)
Pages (from-to)1603-1606
Number of pages4
JournalBlood
Volume110
Issue number5
DOIs
StatePublished - Sep 1 2007

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T-cells
Aplastic Anemia
Regulatory T-Lymphocytes
T-Lymphocytes
NFATC Transcription Factors
Autoimmunity
Autoimmune Diseases
Transfection
Plasmids
Homeostasis
Transcription Factors
Bone Marrow
Messenger RNA
Defects
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Solomou, E. E., Rezvani, K., Mielke, S., Malide, D., Keyvanfar, K., Visconte, V., ... Young, N. S. (2007). Deficient CD4+ CD25+ FOXP3+ T regulatory cells in acquired aplastic anemia. Blood, 110(5), 1603-1606. https://doi.org/10.1182/blood-2007-01-066258

Deficient CD4+ CD25+ FOXP3+ T regulatory cells in acquired aplastic anemia. / Solomou, Elena E.; Rezvani, Katayoun; Mielke, Stephan; Malide, Daniela; Keyvanfar, Keyvan; Visconte, Valeria; Kajigaya, Sachiko; Barrett, A. John; Young, Neal S.

In: Blood, Vol. 110, No. 5, 01.09.2007, p. 1603-1606.

Research output: Contribution to journalArticle

Solomou, EE, Rezvani, K, Mielke, S, Malide, D, Keyvanfar, K, Visconte, V, Kajigaya, S, Barrett, AJ & Young, NS 2007, 'Deficient CD4+ CD25+ FOXP3+ T regulatory cells in acquired aplastic anemia', Blood, vol. 110, no. 5, pp. 1603-1606. https://doi.org/10.1182/blood-2007-01-066258
Solomou EE, Rezvani K, Mielke S, Malide D, Keyvanfar K, Visconte V et al. Deficient CD4+ CD25+ FOXP3+ T regulatory cells in acquired aplastic anemia. Blood. 2007 Sep 1;110(5):1603-1606. https://doi.org/10.1182/blood-2007-01-066258
Solomou, Elena E. ; Rezvani, Katayoun ; Mielke, Stephan ; Malide, Daniela ; Keyvanfar, Keyvan ; Visconte, Valeria ; Kajigaya, Sachiko ; Barrett, A. John ; Young, Neal S. / Deficient CD4+ CD25+ FOXP3+ T regulatory cells in acquired aplastic anemia. In: Blood. 2007 ; Vol. 110, No. 5. pp. 1603-1606.
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