Deficient expression of enhanced reactivation of parvovirus H-1 in ataxia telangiectasia cells irradiated with X-rays or u.v. light

Geneviève Hilgers, Yong Q. Chen, Jan J. Cornelis, Jean Rommelaere

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cells of patients with ataxia telangiectasia (AT), an inherited disease characterized by a high propensity to cancer, are hypersensitive to ionizing radiation. We investigated whether the hyper-radiosensitivity of AT cells correlated with a defect in their constitutive and/or conditional ability to rescue a damaged exogenous virus. For that purpose, parvovirus H-1, a single-stranded DNA virus whose intranuclear replication mostly relies on host cell functions, was used as a probe. The survival of u.v.-or γ-irradiated H-1 was measured in X-, u.v.- or mock-irradiated human cells of normal (NB-E) or AT (AT5BIVA) origin. γ-irradiated H-1 survived to similar extents in untreated normal and AT cell lines. Both X- and u.v.-irradiatlon induced normal cells to achieve an enhanced reactivation (ER) of -γ- or u.v.-damaged H-1. In contrast, neither dose-effect curves nor time course revealed significant levels of ER expression after X- or u.v.-irradiation in AT5BIVA cells. Our results suggest that the impairment of ER of damaged parvoviruses may constitute a marker of the AT cell phenotype and be related to the radiosensitivity of AT cells.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalCarcinogenesis
Volume8
Issue number2
DOIs
StatePublished - Feb 1 1987

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H-1 parvovirus
Ataxia Telangiectasia
X-Rays
Light
Radiation Tolerance
Parvovirus
DNA Viruses
Single-Stranded DNA
Ionizing Radiation

ASJC Scopus subject areas

  • Cancer Research

Cite this

Deficient expression of enhanced reactivation of parvovirus H-1 in ataxia telangiectasia cells irradiated with X-rays or u.v. light. / Hilgers, Geneviève; Chen, Yong Q.; Cornelis, Jan J.; Rommelaere, Jean.

In: Carcinogenesis, Vol. 8, No. 2, 01.02.1987, p. 315-319.

Research output: Contribution to journalArticle

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