Dendritic cell-activated cytokine-induced killer cells enhance the anti-tumor effect of chemotherapy on non-small cell lung cancer in patients after surgery

Hui Li, Changli Wang, Jinpu Yu, Shui Cao, Feng Wei, Weihong Zhang, Ying Han, Xiubao Ren

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    Abstract

    Background aims Cytokine-induced killer (CIK) cells have shown cytolytic activity against several tumor cells in vitro and in animal tumor models. Furthermore, CIK cells activated by dendritic cell (DC) stimulation show increased anti-tumor activity. This study aimed to evaluate the clinical efficacy of DC-activated CIK cell treatment following regular chemotherapy and the effects of this therapy on immune responses in patients with non-small cell lung cancer (NSCLC) after surgery. Methods A paired study, with 42 patients in each group with stage IIIIa NSCLC after surgery, was performed. Patients received chemotherapy alone (CT) or chemotherapy and DC-activated CIK cell treatment (immuno-CT). Disease-free survival (DFS) and overall survival were evaluated. CIK cell cytotoxicity against tumor cells was detected using a lactate dehydrogenase-based method. Serum cytokine levels in the immuno-CT group were detected using cytokine antibody arrays. Results The cytotoxicity of CIK cells was significantly enhanced by DC activation. The 2-year overall survival rate in the immuno-CT group was significantly improved compared with the CT group (94.7 ± 3.6% versus 78.8 ± 7.0%, P < 0.05). The 2-year DFS of these two groups showed no significant difference. DC-activated CIK cell treatment increased production of cytokines that have known anti-tumor effects, including IFN-γ, MIG, TNF-α and TNF-β, in patients who had no progression, but they were not found in patients who developed recurrence/metastasis. Conclusions This study suggests that the role of DC-activated CIK cells in improvement of chemotherapy for malignant tumor treatment is associated with up-regulation of the production of cytokines involved in the anti-tumor effect.

    Original languageEnglish (US)
    Pages (from-to)1076-1083
    Number of pages8
    JournalCytotherapy
    Volume11
    Issue number8
    DOIs
    StatePublished - Jan 1 2009

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    Keywords

    • Chemotherapy
    • Cytokine-induced killer cells
    • Dendritic cells
    • Non-small cell lung cancer.

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Oncology
    • Genetics(clinical)
    • Cell Biology
    • Transplantation
    • Cancer Research

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