@inbook{78cfe4841b1148659d6e4425f6207f7c,
title = "Detection and quantification of protein spots by pinnacle",
abstract = "Accurate spot detection and quantification is a challenging task that must be performed effectively in order to properly extract the proteomic information from two-dimensional (2-D) gel electrophoresis images. In Morris et al., Bioinformatics 24:529–536, 2008, we introduced Pinnacle, an automatic, fast, effective noncommercial package for spot detection and quantification for 2-D gel images, and subsequently we have developed a freely available gui-based interface for applying the method to a set of gels. In this chapter, we overview Pinnacle, and in a step-by-step manner we describe how to use the software to obtain spot lists and quantifications, to be used for comparative proteomic analysis.",
keywords = "Automated methods, Image processing, Spot detection, Wavelet denoising",
author = "Morris, {Jeffrey S.} and Gutstein, {Howard B.}",
note = "Publisher Copyright: {\textcopyright} Springer Science{\th}Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3255-9_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "185--201",
booktitle = "Methods in Molecular Biology",
}