Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

Kakoli Banerjee, Gita Sharma, S. Upadhyay, B. S. Anand, G. S. Raju, P. S. Khandekar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A DNA hybridization assay was developed using a cloned hepatitis B viral genome to detect the presence of infectious virions in human serum. The merit of this assay was to put in evidence virus particles in 7 out of 133 sera that were negative for surface antigen (HBsAg) using routine serological methods. The usefulness of this assay was confirmed by actual visualization of the virus under electron microscope. Some serum samples although positive for surface antigen, did not give a hybridization signal by dot blot assay and might indicate cases of acute hepatitis

Original languageEnglish (US)
Pages (from-to)279-287
Number of pages9
JournalJournal of Biosciences
Volume14
Issue number3
DOIs
StatePublished - Sep 1 1989

Fingerprint

Hepatitis B virus
DNA probes
surface antigens
DNA Probes
Surface Antigens
Viruses
Assays
Blood
hybridization
blood serum
Virion
blood
assays
Serum
virion
Viral Genome
Hepatitis B Surface Antigens
Hepatitis B
sampling
Hepatitis

Keywords

  • DNA dot hybridization
  • hepatitis B virus
  • integration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay. / Banerjee, Kakoli; Sharma, Gita; Upadhyay, S.; Anand, B. S.; Raju, G. S.; Khandekar, P. S.

In: Journal of Biosciences, Vol. 14, No. 3, 01.09.1989, p. 279-287.

Research output: Contribution to journalArticle

Banerjee, Kakoli ; Sharma, Gita ; Upadhyay, S. ; Anand, B. S. ; Raju, G. S. ; Khandekar, P. S. / Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay. In: Journal of Biosciences. 1989 ; Vol. 14, No. 3. pp. 279-287.
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