Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

Kakoli Banerjee; Gita Sharma; S. Upadhyay; B. S. Anand; G. S. Raju; P. S. Khandekar

doi:10.1007/BF02716689

Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

Kakoli Banerjee, Gita Sharma, S. Upadhyay, B. S. Anand, G. S. Raju, P. S. Khandekar

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A DNA hybridization assay was developed using a cloned hepatitis B viral genome to detect the presence of infectious virions in human serum. The merit of this assay was to put in evidence virus particles in 7 out of 133 sera that were negative for surface antigen (HBsAg) using routine serological methods. The usefulness of this assay was confirmed by actual visualization of the virus under electron microscope. Some serum samples although positive for surface antigen, did not give a hybridization signal by dot blot assay and might indicate cases of acute hepatitis

Original language	English (US)
Pages (from-to)	279-287
Number of pages	9
Journal	Journal of Biosciences
Volume	14
Issue number	3
DOIs	https://doi.org/10.1007/BF02716689
State	Published - Sep 1989
Externally published	Yes

Keywords

DNA dot hybridization
hepatitis B virus
integration

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.1007/BF02716689

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title = "Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay",

abstract = "A DNA hybridization assay was developed using a cloned hepatitis B viral genome to detect the presence of infectious virions in human serum. The merit of this assay was to put in evidence virus particles in 7 out of 133 sera that were negative for surface antigen (HBsAg) using routine serological methods. The usefulness of this assay was confirmed by actual visualization of the virus under electron microscope. Some serum samples although positive for surface antigen, did not give a hybridization signal by dot blot assay and might indicate cases of acute hepatitis",

keywords = "DNA dot hybridization, hepatitis B virus, integration",

author = "Kakoli Banerjee and Gita Sharma and S. Upadhyay and Anand, {B. S.} and Raju, {G. S.} and Khandekar, {P. S.}",

year = "1989",

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doi = "10.1007/BF02716689",

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T1 - Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

AU - Banerjee, Kakoli

AU - Sharma, Gita

AU - Upadhyay, S.

AU - Anand, B. S.

AU - Raju, G. S.

AU - Khandekar, P. S.

PY - 1989/9

Y1 - 1989/9

N2 - A DNA hybridization assay was developed using a cloned hepatitis B viral genome to detect the presence of infectious virions in human serum. The merit of this assay was to put in evidence virus particles in 7 out of 133 sera that were negative for surface antigen (HBsAg) using routine serological methods. The usefulness of this assay was confirmed by actual visualization of the virus under electron microscope. Some serum samples although positive for surface antigen, did not give a hybridization signal by dot blot assay and might indicate cases of acute hepatitis

AB - A DNA hybridization assay was developed using a cloned hepatitis B viral genome to detect the presence of infectious virions in human serum. The merit of this assay was to put in evidence virus particles in 7 out of 133 sera that were negative for surface antigen (HBsAg) using routine serological methods. The usefulness of this assay was confirmed by actual visualization of the virus under electron microscope. Some serum samples although positive for surface antigen, did not give a hybridization signal by dot blot assay and might indicate cases of acute hepatitis

KW - DNA dot hybridization

KW - hepatitis B virus

KW - integration

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IS - 3

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Detection of hepatitis B virus in blood samples negative for surface antigen, by DNA probe hybridization assay

Abstract

Keywords

ASJC Scopus subject areas

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