Determinación del contenido de ADN mediante citofotometría en suspensión nuclear conservada por períodos prolongados (estudio preliminar).

I. Roa; J. C. Araya; I. Wistuba; M. Villaseca; X. de Aretxabala; A. Ferreira; A. Gómez

Determinación del contenido de ADN mediante citofotometría en suspensión nuclear conservada por períodos prolongados (estudio preliminar).

Translated title of the contribution: Determination of DNA content using cytophotometry in nuclear suspension conserved for prolonged periods (preliminary study)

I. Roa
, J. C. Araya
, I. Wistuba
, M. Villaseca
, X. de Aretxabala
, A. Ferreira
, A. Gómez

Research output: Contribution to journal › Article › peer-review

Abstract

The variations in cytophotometric determination of DNA content were studied in a concentrated suspension of nuclei stored for prolonged periods. These came from normal and tumoral cells of an uterine leiomyosarcoma, fixed in formalin and included in paraffin. DNA was measured in normal and tumoral cells at days 0, 1, 7, 11, 19, 27 and 33. No significant variations in DNA measurement were observed. In a same day, there was an uniform DNA content, ploidy pattern and DNA index and an aneuploid pattern was diagnosed in all histograms. These results suggest that nuclear suspensions can be conserved for long periods and used for DNA content determination, allowing the access of remote places to these techniques.

Translated title of the contribution	Determination of DNA content using cytophotometry in nuclear suspension conserved for prolonged periods (preliminary study)
Original language	Spanish
Pages (from-to)	253-258
Number of pages	6
Journal	Revista médica de Chile
Volume	122
Issue number	3
State	Published - Mar 1994

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Determinaci{\'o}n del contenido de ADN mediante citofotometr{\'i}a en suspensi{\'o}n nuclear conservada por per{\'i}odos prolongados (estudio preliminar).",

abstract = "The variations in cytophotometric determination of DNA content were studied in a concentrated suspension of nuclei stored for prolonged periods. These came from normal and tumoral cells of an uterine leiomyosarcoma, fixed in formalin and included in paraffin. DNA was measured in normal and tumoral cells at days 0, 1, 7, 11, 19, 27 and 33. No significant variations in DNA measurement were observed. In a same day, there was an uniform DNA content, ploidy pattern and DNA index and an aneuploid pattern was diagnosed in all histograms. These results suggest that nuclear suspensions can be conserved for long periods and used for DNA content determination, allowing the access of remote places to these techniques.",

author = "I. Roa and Araya, \{J. C.\} and I. Wistuba and M. Villaseca and \{de Aretxabala\}, X. and A. Ferreira and A. G{\'o}mez",

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language = "Spanish",

volume = "122",

pages = "253--258",

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AU - Roa, I.

AU - Araya, J. C.

AU - Wistuba, I.

AU - Villaseca, M.

AU - de Aretxabala, X.

AU - Ferreira, A.

AU - Gómez, A.

N1 - Copyright: This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

PY - 1994/3

Y1 - 1994/3

N2 - The variations in cytophotometric determination of DNA content were studied in a concentrated suspension of nuclei stored for prolonged periods. These came from normal and tumoral cells of an uterine leiomyosarcoma, fixed in formalin and included in paraffin. DNA was measured in normal and tumoral cells at days 0, 1, 7, 11, 19, 27 and 33. No significant variations in DNA measurement were observed. In a same day, there was an uniform DNA content, ploidy pattern and DNA index and an aneuploid pattern was diagnosed in all histograms. These results suggest that nuclear suspensions can be conserved for long periods and used for DNA content determination, allowing the access of remote places to these techniques.

AB - The variations in cytophotometric determination of DNA content were studied in a concentrated suspension of nuclei stored for prolonged periods. These came from normal and tumoral cells of an uterine leiomyosarcoma, fixed in formalin and included in paraffin. DNA was measured in normal and tumoral cells at days 0, 1, 7, 11, 19, 27 and 33. No significant variations in DNA measurement were observed. In a same day, there was an uniform DNA content, ploidy pattern and DNA index and an aneuploid pattern was diagnosed in all histograms. These results suggest that nuclear suspensions can be conserved for long periods and used for DNA content determination, allowing the access of remote places to these techniques.

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M3 - Article

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AN - SCOPUS:0028402341

SN - 0034-9887

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JF - Revista médica de Chile

IS - 3

ER -

Determinación del contenido de ADN mediante citofotometría en suspensión nuclear conservada por períodos prolongados (estudio preliminar).

Abstract

ASJC Scopus subject areas

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