TY - GEN
T1 - Developing hyperpolarized silicon particles for advanced biomedical imaging applications
AU - Whiting, Nicholas
AU - Hu, Jingzhe
AU - Constantinou, Pamela
AU - Millward, Niki Zacharias
AU - Bankson, James
AU - Gorenstein, David
AU - Sood, Anil
AU - Carson, Daniel
AU - Bhattacharya, Pratip
N1 - Publisher Copyright:
© 2015 SPIE.
PY - 2015
Y1 - 2015
N2 - Silicon-based nanoparticles are ideally suited as biomedical imaging agents, due to their biocompatibility, biodegradability, and simple surface chemistry that is amenable to drug loading and targeting. A method of hyperpolarizing silicon particles using dynamic nuclear polarization (DNP), which increases magnetic resonance imaging (MRI) signals by 4-5 orders of magnitude through enhanced nuclear spin alignment, has recently been developed and shown viable as a contrast agent for in vivo MRI. Naturally occurring electronic defects on the particle surface obviate the need for exogenous radicals, and the enhanced spin polarization lasts for significantly longer than other hyperpolarized agents (tens of minutes, instead of <1 minute for other species). We report our recent advances in determining the MR characteristics of hyperpolarized silicon particles, which could lead to non-invasive, non-radioactive molecular targeted imaging of various cancer systems. A variety of particle sizes (20 nm-2 μm) were found to have hyperpolarized relaxation times ranging from ∼10-50 minutes. The addition of various functional groups to the particle surface, including biocompatible polymers, aptamers, and antibodies had no effect to the hyperpolarization dynamics or relaxation times, and appear to satisfactorily survive the harsh temperature conditions of DNP. Preliminary in vivo studies examined a variety of particle administration routes in mice, including intraperitoneal, tail vein, and rectal injections, as well as oral gavage. Ongoing experiments include targeted molecular imaging in orthotopic murine models of ovarian and colorectal cancers.
AB - Silicon-based nanoparticles are ideally suited as biomedical imaging agents, due to their biocompatibility, biodegradability, and simple surface chemistry that is amenable to drug loading and targeting. A method of hyperpolarizing silicon particles using dynamic nuclear polarization (DNP), which increases magnetic resonance imaging (MRI) signals by 4-5 orders of magnitude through enhanced nuclear spin alignment, has recently been developed and shown viable as a contrast agent for in vivo MRI. Naturally occurring electronic defects on the particle surface obviate the need for exogenous radicals, and the enhanced spin polarization lasts for significantly longer than other hyperpolarized agents (tens of minutes, instead of <1 minute for other species). We report our recent advances in determining the MR characteristics of hyperpolarized silicon particles, which could lead to non-invasive, non-radioactive molecular targeted imaging of various cancer systems. A variety of particle sizes (20 nm-2 μm) were found to have hyperpolarized relaxation times ranging from ∼10-50 minutes. The addition of various functional groups to the particle surface, including biocompatible polymers, aptamers, and antibodies had no effect to the hyperpolarization dynamics or relaxation times, and appear to satisfactorily survive the harsh temperature conditions of DNP. Preliminary in vivo studies examined a variety of particle administration routes in mice, including intraperitoneal, tail vein, and rectal injections, as well as oral gavage. Ongoing experiments include targeted molecular imaging in orthotopic murine models of ovarian and colorectal cancers.
KW - Hyperpolarization
KW - MRI
KW - Molecular imaging
KW - Nanomedicine
KW - Silicon particles
UR - http://www.scopus.com/inward/record.url?scp=84943370994&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943370994&partnerID=8YFLogxK
U2 - 10.1117/12.2082252
DO - 10.1117/12.2082252
M3 - Conference contribution
AN - SCOPUS:84943370994
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Medical Imaging 2015
A2 - Gimi, Barjor
A2 - Molthen, Robert C.
PB - SPIE
T2 - Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging
Y2 - 24 February 2015 through 26 February 2015
ER -