Different metastasis patterns of a human melanoma cell line in nude mice and rats:Influence of microenvironment

Inge Kjønniksen, Hanne Kleppe Høifødt, Alexander Pihl, øystein Fodstad

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The metastatic capacity of intravenously injected human FEMX-I melanoma cells in athymic nude mice and rats was compared. Young rats given 1 x 10 6 ascites tumor cells all died of lung tumors with a life span of 50 ± 10 days (mean ± SD). In contrast, in accordance with previous findings, only extrapulmonary metastases developed in mice. This host-dependent difference in metastasis pattern permitted studies on the role of factors that may influence the organ specificity of metastases. The tissue distribution of 125-labeled FEMX-I cells did not differ in the two nude species during the first 12 hours after cell injection. The plating efficiency of FEMX-I cells in soft agar was increased by the addition of conditioned medium prepared from rat lungs, resulting also in a significant increase in colony size. In contrast, conditioned medium prepared from mouse lungs reduced the clonogenic capacity of the FEMX-I cells in a dose-dependent manner. Conditioned media prepared from rat and mouse liver, kidney, and spleen tissues either inhibited or had no effect on colony formation. The results suggest that the unexpected differential metastatic patterns observed in vivo may reflect differences in the presence of growth-modulating paracrine factors in the host lungs. [J Natl Cancer Inst 83: 1020-1024, 1991]

Original languageEnglish (US)
Pages (from-to)1020-1024
Number of pages5
JournalJournal of the National Cancer Institute
Volume83
Issue number14
DOIs
StatePublished - Jul 17 1991

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Nude Rats
Nude Mice
Melanoma
Neoplasm Metastasis
Cell Line
Conditioned Culture Medium
Lung
Neoplasms
Organ Specificity
Tissue Distribution
Ascites
Agar
Spleen
Kidney
Injections
Liver
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Different metastasis patterns of a human melanoma cell line in nude mice and rats:Influence of microenvironment. / Kjønniksen, Inge; Høifødt, Hanne Kleppe; Pihl, Alexander; Fodstad, øystein.

In: Journal of the National Cancer Institute, Vol. 83, No. 14, 17.07.1991, p. 1020-1024.

Research output: Contribution to journalArticle

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