TY - JOUR
T1 - Differentiating effect of l-tyrosine on the human melanoma cell line IIB-MEL-J
AU - Guerra, Liliana
AU - Bover, Laura
AU - Mordoh, Jose
N1 - Funding Information:
This work has been supported by grants from the Consejo National de Investigaciones Cientificas y Tecnicas (CONICET), the Facultad de Ciencias Exactas y Naturales de la Universidad de Buenos Aires, the Fundacion Maria Calderon de la Barca, and the Fundacion para la Investigation de1 Cancer (FUCA). L.G. is a Fellow and J.M. is a Career Investigator of the CONICET. We are grateful to Dr. A. N. Houghton for the provision of the R24 monoclonal antibody.
PY - 1990/5
Y1 - 1990/5
N2 - IIB-MEL-J is a highly heterogeneous newly established human melanoma cell line. The addition of 3 mM, l-tyrosine to the culture medium produced (1) a great decrease in the cell growth rate, (2) a loss of the anchorage-independent growth capacity, and (3) a change in the morphology of the cells to a fibroblastoid aspect. Coincident with these changes, an increase in subpopulations I and II and a decrease in subpopulations III and IV took place. In view of this evidence we consider that the cells have differentiated. The melanin production was not increased by the l-tyrosine treatment, suggesting that differentiation and melanin expression are not strictly correlated.
AB - IIB-MEL-J is a highly heterogeneous newly established human melanoma cell line. The addition of 3 mM, l-tyrosine to the culture medium produced (1) a great decrease in the cell growth rate, (2) a loss of the anchorage-independent growth capacity, and (3) a change in the morphology of the cells to a fibroblastoid aspect. Coincident with these changes, an increase in subpopulations I and II and a decrease in subpopulations III and IV took place. In view of this evidence we consider that the cells have differentiated. The melanin production was not increased by the l-tyrosine treatment, suggesting that differentiation and melanin expression are not strictly correlated.
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U2 - 10.1016/0014-4827(90)90278-I
DO - 10.1016/0014-4827(90)90278-I
M3 - Article
C2 - 2328778
AN - SCOPUS:0025240033
SN - 0014-4827
VL - 188
SP - 61
EP - 65
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -