@inbook{e16b0d8bd24747f6ae8ab71f236d3fc0,
title = "Digital PCR: Principles and applications",
abstract = "Digital PCR is a robust PCR technique that enables precise and accurate absolute quantitation of target molecules by diluting and partitioning of the samples into numerous compartments. Automated partitioning can be attained by creating {\textquotedblleft}water-in-oil{\textquotedblright} emulsion (emulsion-based digital PCR) or using a chip with microchannels (microfluidics-based digital PCR). We discuss the advantages and a wide variety of clinical applications of this technique. We describe the droplet digital RT-PCR protocol published by Jennings et al. for identification and absolute quantitation of BCR-ABL1 transcripts.",
keywords = "BCR-ABL1 transcript, Droplet digital PCR, Emulsion PCR, Microfluidics",
author = "Rashmi Kanagal-Shamanna",
year = "2016",
month = feb,
day = "1",
doi = "10.1007/978-1-4939-3360-0\_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "43--50",
booktitle = "Methods in Molecular Biology",
}