Discovering functional ERK substrates regulating Caenorhabditis elegans Germline development

Jessica Jie Chen, Swathi Arur

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signaling has been shown to regulate over seven distinct biological processes in a temporal and sequential manner. To fully elucidate how ERK signaling cascade orchestrates these different biological processes in vivo, identifi cation of the direct functional substrates of the pathway is critical. This chapter describes the methods that were used to identify ERK substrates in a global manner and study their functions in the germline. These approaches can also be generally applied to study ERK-dependent biological processes in other systems.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages317-335
Number of pages19
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1487
ISSN (Print)1064-3745

Keywords

  • C. elegans oogenesis
  • ERK substrates
  • Functional genomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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