DNA curvature in solution measured by fluorescence resonance energy transfer

Katalin Tóth, Volker Sauermann, Jörg Langowski

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The sequence-induced curvature of DNA fragments free in solution was characterized by measurements of the end-to-end distance using fluorescence resonance energy transfer (FRET). The 31 bp oligonucleotides were labeled at their 5' ends with fluorescein as the donor and rhodamine X as the acceptor. We compared a curved oligonucleotide with three phased A6 blocks and a control containing (AT)3 instead of the A6 blocks. The increased efficiency of energy transfer of the A6-containing DNA indicates the existence of a permanent sequence-induced curvature, the magnitude of which is in good agreement with estimates from theoretical curvature predictions. Energy transfer efficiency and correspondingly curvature increases with NaCl concentration.

Original languageEnglish (US)
Pages (from-to)8173-8179
Number of pages7
JournalBiochemistry
Volume37
Issue number22
DOIs
StatePublished - Jun 2 1998

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Fluorescence Resonance Energy Transfer
Energy Transfer
Oligonucleotides
Energy transfer
Rhodamines
DNA
Fluorescein

ASJC Scopus subject areas

  • Biochemistry

Cite this

DNA curvature in solution measured by fluorescence resonance energy transfer. / Tóth, Katalin; Sauermann, Volker; Langowski, Jörg.

In: Biochemistry, Vol. 37, No. 22, 02.06.1998, p. 8173-8179.

Research output: Contribution to journalArticle

Tóth, Katalin ; Sauermann, Volker ; Langowski, Jörg. / DNA curvature in solution measured by fluorescence resonance energy transfer. In: Biochemistry. 1998 ; Vol. 37, No. 22. pp. 8173-8179.
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