DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo

James R. Jabbur; Peng Huang; Wei Zhang

doi:10.1038/sj.onc.1204017

DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo

James R. Jabbur
, Peng Huang
, Wei Zhang

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV). A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20. Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV. The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21^WAF1/Cip1 (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo.

Original language	English (US)
Pages (from-to)	6203-6208
Number of pages	6
Journal	Oncogene
Volume	19
Issue number	54
DOIs	https://doi.org/10.1038/sj.onc.1204017
State	Published - Dec 14 2000

Keywords

Ionizing radiation
Phosphorylation
p21
p53

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1038/sj.onc.1204017

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title = "DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo",

abstract = "We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV). A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20. Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV. The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21WAF1/Cip1 (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo.",

keywords = "Ionizing radiation, Phosphorylation, p21, p53",

author = "Jabbur, \{James R.\} and Peng Huang and Wei Zhang",

note = "Funding Information: We thank Drs Gigi Lozano, Paul Chiao, Raymond Meyn, Ralph Arlinghaus, Tse-Hua Tan, Elizabeth Grimm and Lei Li for their helpful discussions. We thank Lore Feldman for her editorial assistance. This work was supported in part by National Institutes of Health grants CA67987, CA55164 and CA77339. The MD Anderson sequencing core facility is supported by Core Grant CA16672. JR Jabbur was a recipient of the American Legion Auxiliary Fellowship.",

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doi = "10.1038/sj.onc.1204017",

language = "English (US)",

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T1 - DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo

AU - Jabbur, James R.

AU - Huang, Peng

AU - Zhang, Wei

N1 - Funding Information: We thank Drs Gigi Lozano, Paul Chiao, Raymond Meyn, Ralph Arlinghaus, Tse-Hua Tan, Elizabeth Grimm and Lei Li for their helpful discussions. We thank Lore Feldman for her editorial assistance. This work was supported in part by National Institutes of Health grants CA67987, CA55164 and CA77339. The MD Anderson sequencing core facility is supported by Core Grant CA16672. JR Jabbur was a recipient of the American Legion Auxiliary Fellowship.

PY - 2000/12/14

Y1 - 2000/12/14

N2 - We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV). A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20. Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV. The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21WAF1/Cip1 (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo.

AB - We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV). A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20. Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV. The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21WAF1/Cip1 (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo.

KW - Ionizing radiation

KW - Phosphorylation

KW - p21

KW - p53

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DO - 10.1038/sj.onc.1204017

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AN - SCOPUS:0034649511

SN - 0950-9232

VL - 19

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JO - Oncogene

JF - Oncogene

IS - 54

ER -

DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo

Abstract

Keywords

ASJC Scopus subject areas

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