Downregulation of c-myc RNA is not a prerequisite for reduced cell proliferation, but is associated with G1 arrest in B-lymphoid cell lines

Jon Lømo, Harald Holte, Catharina de Lange Davies, Erik Ruud, Monica Laukas, Erlend B. Smeland, Tore Godal, Heidi Kiil Blomhoff

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We related the effects of c-myc expression on the ability of growth inhibitors to block the cells in the G0 G1 phase of the cell cycle. In two different B-cell lines, there was an association between the accumulation of cells in the middle to late G1 phase of the cell cycle and a rapid transient downregulation of c-myc mRNA levels. The phorbol ester TPA and the adenylate cyclase activator forskolin reduced the c-myc RNA levels, and after 3 days of treatment a proportion of the cells accumulated in G1. In contrast, neither interferon-γ, tumor necrosis factor-α nor the monoclonal antibody 33-1 against DQ major histocompatibility antigens changed the cell-cycle distribution or regulated the c-myc RNA levels. Yet, all five growth inhibitors reduced the proliferation to approximately the same extent. The growth reduction was not accompanied by definite differentiation, as judged by the absence of the B-cell differentiation marker B1 (CD20).

Original languageEnglish (US)
Pages (from-to)84-91
Number of pages8
JournalExperimental Cell Research
Volume172
Issue number1
DOIs
StatePublished - Jan 1 1987

Fingerprint

Growth Inhibitors
Cell Cycle
Down-Regulation
Cell Proliferation
G1 Phase
Lymphocytes
RNA
Cell Line
B-Lymphocytes
Cell Cycle Resting Phase
Histocompatibility Antigens
Differentiation Antigens
Phorbol Esters
Colforsin
Adenylyl Cyclases
Interferons
Cell Differentiation
Tumor Necrosis Factor-alpha
Monoclonal Antibodies
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Downregulation of c-myc RNA is not a prerequisite for reduced cell proliferation, but is associated with G1 arrest in B-lymphoid cell lines. / Lømo, Jon; Holte, Harald; de Lange Davies, Catharina; Ruud, Erik; Laukas, Monica; Smeland, Erlend B.; Godal, Tore; Blomhoff, Heidi Kiil.

In: Experimental Cell Research, Vol. 172, No. 1, 01.01.1987, p. 84-91.

Research output: Contribution to journalArticle

Lømo, Jon ; Holte, Harald ; de Lange Davies, Catharina ; Ruud, Erik ; Laukas, Monica ; Smeland, Erlend B. ; Godal, Tore ; Blomhoff, Heidi Kiil. / Downregulation of c-myc RNA is not a prerequisite for reduced cell proliferation, but is associated with G1 arrest in B-lymphoid cell lines. In: Experimental Cell Research. 1987 ; Vol. 172, No. 1. pp. 84-91.
@article{27b0a03cc9a448228bb8866146019fc6,
title = "Downregulation of c-myc RNA is not a prerequisite for reduced cell proliferation, but is associated with G1 arrest in B-lymphoid cell lines",
abstract = "We related the effects of c-myc expression on the ability of growth inhibitors to block the cells in the G0 G1 phase of the cell cycle. In two different B-cell lines, there was an association between the accumulation of cells in the middle to late G1 phase of the cell cycle and a rapid transient downregulation of c-myc mRNA levels. The phorbol ester TPA and the adenylate cyclase activator forskolin reduced the c-myc RNA levels, and after 3 days of treatment a proportion of the cells accumulated in G1. In contrast, neither interferon-γ, tumor necrosis factor-α nor the monoclonal antibody 33-1 against DQ major histocompatibility antigens changed the cell-cycle distribution or regulated the c-myc RNA levels. Yet, all five growth inhibitors reduced the proliferation to approximately the same extent. The growth reduction was not accompanied by definite differentiation, as judged by the absence of the B-cell differentiation marker B1 (CD20).",
author = "Jon L{\o}mo and Harald Holte and {de Lange Davies}, Catharina and Erik Ruud and Monica Laukas and Smeland, {Erlend B.} and Tore Godal and Blomhoff, {Heidi Kiil}",
year = "1987",
month = "1",
day = "1",
doi = "10.1016/0014-4827(87)90095-4",
language = "English (US)",
volume = "172",
pages = "84--91",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Downregulation of c-myc RNA is not a prerequisite for reduced cell proliferation, but is associated with G1 arrest in B-lymphoid cell lines

AU - Lømo, Jon

AU - Holte, Harald

AU - de Lange Davies, Catharina

AU - Ruud, Erik

AU - Laukas, Monica

AU - Smeland, Erlend B.

AU - Godal, Tore

AU - Blomhoff, Heidi Kiil

PY - 1987/1/1

Y1 - 1987/1/1

N2 - We related the effects of c-myc expression on the ability of growth inhibitors to block the cells in the G0 G1 phase of the cell cycle. In two different B-cell lines, there was an association between the accumulation of cells in the middle to late G1 phase of the cell cycle and a rapid transient downregulation of c-myc mRNA levels. The phorbol ester TPA and the adenylate cyclase activator forskolin reduced the c-myc RNA levels, and after 3 days of treatment a proportion of the cells accumulated in G1. In contrast, neither interferon-γ, tumor necrosis factor-α nor the monoclonal antibody 33-1 against DQ major histocompatibility antigens changed the cell-cycle distribution or regulated the c-myc RNA levels. Yet, all five growth inhibitors reduced the proliferation to approximately the same extent. The growth reduction was not accompanied by definite differentiation, as judged by the absence of the B-cell differentiation marker B1 (CD20).

AB - We related the effects of c-myc expression on the ability of growth inhibitors to block the cells in the G0 G1 phase of the cell cycle. In two different B-cell lines, there was an association between the accumulation of cells in the middle to late G1 phase of the cell cycle and a rapid transient downregulation of c-myc mRNA levels. The phorbol ester TPA and the adenylate cyclase activator forskolin reduced the c-myc RNA levels, and after 3 days of treatment a proportion of the cells accumulated in G1. In contrast, neither interferon-γ, tumor necrosis factor-α nor the monoclonal antibody 33-1 against DQ major histocompatibility antigens changed the cell-cycle distribution or regulated the c-myc RNA levels. Yet, all five growth inhibitors reduced the proliferation to approximately the same extent. The growth reduction was not accompanied by definite differentiation, as judged by the absence of the B-cell differentiation marker B1 (CD20).

UR - http://www.scopus.com/inward/record.url?scp=0023604549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023604549&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(87)90095-4

DO - 10.1016/0014-4827(87)90095-4

M3 - Article

C2 - 3115797

AN - SCOPUS:0023604549

VL - 172

SP - 84

EP - 91

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -