Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo

Yanyan Ma, Zengtao Wei, Robert C. Bast, Zhanying Wang, Yan Li, Meng Gao, Yanping Liu, Chun Guo, Lining Zhang, Xiaoyan Wang

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

TRIM27 (tripartite motif-containing 27) was originally identified as a fusion partner with the RET (REarranged during transfection) proto-oncogene and is highly expressed in various tumor cells and tissues. However, the level of expression and function of TRIM27 in ovarian cancer remain unclear. Here we have measured the expression of TRIM27 in normal ovarian and fallopian tube epithelial cells and in ovarian serous carcinoma cells and correlated TRIM27 expression with clinical and pathological parameters. In addition, we detected the effect of TRIM27 knockdown on proliferation of ovarian cancer cells in cell culture and xenografts. The results demonstrated that TRIM27 was highly expressed in ovarian serous carcinoma cells, and TRIM27 expression was significantly correlated with metastasis and FIGO stage in ovarian serous carcinoma patients. Downregulation of TRIM27 expression suppressed the proliferation of ovarian cancer cells in cell culture and inhibited the growth of xenografts in nude mice. TRIM27 knockdown induced cell cycle arrest and apoptosis in ovarian cancer cells by upregulating the expression of p-P38 and downregulating the expression of p-AKT. Thus the present study suggests that TRIM27 could have important roles as an oncogene during the development of ovarian cancer and could serve as a diagnostic and therapeutic target.

Original languageEnglish (US)
Pages (from-to)37-48
Number of pages12
JournalLaboratory Investigation
Volume96
Issue number1
DOIs
StatePublished - Jan 1 2016

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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