@article{477ffe8ed7ac48a989150c009bc12fd4,
title = "E2F3a stimulates proliferation, p53-independent apoptosis and carcinogenesis in a transgenic mouse model",
abstract = "Mutation or inactivation of the retinoblastoma (Rb) tumor suppressor occurs in most human tumors and results in the deregulation of several members of the E2F family of transcription factors. Among the E2F family, E2F3 has been implicated as a key regulator of cell proliferation and E2F3 gene amplification and overexpression is detected in some human tumors. To study the role of E2F3 in tumor development, we established a transgenic mouse model expressing E2F3a in a number of epithelial tissues via a keratin 5 (K5) promoter. Transgenic expression of E2F3a leads to hyperproliferation, hyperplasia and increased levels of p53-independent apoptosis in transgenic epidermis. Consistent with data from human cancers, the E2F3a transgene is found to have a weak oncogenic activity on its own and to significantly enhance the response to a skin carcinogenesis protocol. The phenotype of K5 E2F3a transgenic mice is distinct from similar transgenic mice expressing E2F1 or E2F4. In particular, E2F3a has a unique apoptotic activity and lacks the tumor suppressive property of E2F1 in this model system.",
keywords = "Apoptosis, Cell cycle, E2F3, Skin carcinogenesis, Transgenic mouse",
author = "Paulson, {Qiwei X.} and McArthur, {Mark J.} and Johnson, {David G.}",
note = "Funding Information: assistance, Penny Riggs for real-time PCR analysis, The E2Fs have been divided into several subclasses according to their transcriptional Dale Weiss, Lezlee Coghlan and coworkers for regulatory properties on model E2F target genes. One subclass includes E2F1, E2F2, and animal care, Irma Gimenez-Conti, Nancy Abbey E2F3a, which are referred to as the activating E2Fs because they transcriptionally activate and coworkers for histology, Chris Brown and Joi E2F target genes such as thymidine kinase and cyclin E. The activating E2Fs associate pre-Holcomb for art work, Howard Thames and Kevin dominately with Rb and are expressed in a cell cycle-regulated manner with maximum Lin for statistical analysis, and Shawnda Sanders for expression in late G1.3 Another subclass of E2Fs, which includes E2F3b, E2F4, and E2F5, preparation of the manuscript. This work was are referred to as repressor E2Fs because their primary function appears to be to repress supported by grants from the National Institutes of the transcription of E2F target genes when in association with Rb, p107, or p130. This CA16672).Health (CA79648 to D.G.J., ES07784 and subclass is expressed constitutively but transcriptional repression by these factors primarily occurs in quiescent and early G1 cells. E2F6, 7 and 8 also appear to function as transcriptional {\textcopyright}2005 LANDES BIOSCIENCE repressors of E2F target genes but do so independent of the Rb family.",
year = "2006",
month = jan,
day = "16",
doi = "10.4161/cc.5.2.2307",
language = "English (US)",
volume = "5",
pages = "184--190",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "2",
}