Abstract
The growth and morphology of 4 tumorigenic rat urothelial cell lines grown on collagen-coated nylon discs was characterized and compared to normal cells. In contrast to cells cultured on a plastic substrate with or without a thin 'nonporous' collagen coating, tumor cells grown on porous collagen-coated nylon discs: 1) grew to greater protein densities; 2) formed tissue structures characteristic for the type of tumor they developed upon back-transplantation; and 3) could be grown and cultured indefinitely without subculturing. Thus, similarly to normal urothelial stratification and differentiation in vitro, tumorigenic cells apparently require a 'porous' collagen substrate to allow differentiation analogous to that observed in vivo.
Original language | English (US) |
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Pages (from-to) | 1211-1216 |
Number of pages | 6 |
Journal | Journal of Urology |
Volume | 130 |
Issue number | 6 |
DOIs | |
State | Published - 1983 |
Externally published | Yes |
ASJC Scopus subject areas
- Urology