Effect of the IgM and IgA secretory tailpieces on polymerization and secretion of IgM and IgG

Vigdis Sørensen, Ingunn B. Rasmussen, Lars Norderhaug, Inger Natvig, Terje E. Michaelsen, Inger Sandlie

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Pentameric IgM and dimeric IgA both contain disulfide bonds between cysteines located in the secretory tailpieces of the heavy chains. To compare the influences of the μ and α tailpieces on the polymeric structure, we have replaced amino acids in the tailpiece of the human μ-chain with amino acids found in the corresponding positions in the human a tailpiece. We show that an IgM with an a tailpiece (IgM L561H, Y562V, L566V, S569A, D570E, TS71V, and A572D) as well as IgM L561H, Y562V, and IgM A572D have a size distribution similar to that of wild-type IgM. However, one IgM mutant with a μ/α hybrid tailpiece (IgM L566V, S569A, D570E, T571V, and A572D) is secreted as a mixture of mainly hexamers, pentamers, tetramers, and dimers. The tetramers and dimers are specifically formed and secreted at the expense of pentamers and hexamers; no alterations in polymerization or secretion rates were observed. We have also incorporated the μ, α, and hybrid μ/α tailpieces to a human IgGS or IgGL309C mutant. The IgG-tailpiece mutants are poorly secreted, but the secreted fractions contain multimeric molecules. Each of the mutants that contain both the L309C mutation and a secretory tailpiece forms mainly hexamers; however, small differences in polymer distribution exist for the different tailpieces. Comparison of the influence of different tailpieces on IgM and IgG polymeric structures suggests that the function of a specific tailpiece is dependent on other parts of the heavy chain, which can vary for different isotypes.

Original languageEnglish (US)
Pages (from-to)2858-2865
Number of pages8
JournalJournal of Immunology
Volume156
Issue number8
StatePublished - Apr 15 1996

Fingerprint

Secretory Immunoglobulin A
Polymerization
Immunoglobulin M
Immunoglobulin G
Amino Acids
secretory IgM
Disulfides
Immunoglobulin A
Cysteine
Polymers
Mutation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sørensen, V., Rasmussen, I. B., Norderhaug, L., Natvig, I., Michaelsen, T. E., & Sandlie, I. (1996). Effect of the IgM and IgA secretory tailpieces on polymerization and secretion of IgM and IgG. Journal of Immunology, 156(8), 2858-2865.

Effect of the IgM and IgA secretory tailpieces on polymerization and secretion of IgM and IgG. / Sørensen, Vigdis; Rasmussen, Ingunn B.; Norderhaug, Lars; Natvig, Inger; Michaelsen, Terje E.; Sandlie, Inger.

In: Journal of Immunology, Vol. 156, No. 8, 15.04.1996, p. 2858-2865.

Research output: Contribution to journalArticle

Sørensen, V, Rasmussen, IB, Norderhaug, L, Natvig, I, Michaelsen, TE & Sandlie, I 1996, 'Effect of the IgM and IgA secretory tailpieces on polymerization and secretion of IgM and IgG', Journal of Immunology, vol. 156, no. 8, pp. 2858-2865.
Sørensen V, Rasmussen IB, Norderhaug L, Natvig I, Michaelsen TE, Sandlie I. Effect of the IgM and IgA secretory tailpieces on polymerization and secretion of IgM and IgG. Journal of Immunology. 1996 Apr 15;156(8):2858-2865.
Sørensen, Vigdis ; Rasmussen, Ingunn B. ; Norderhaug, Lars ; Natvig, Inger ; Michaelsen, Terje E. ; Sandlie, Inger. / Effect of the IgM and IgA secretory tailpieces on polymerization and secretion of IgM and IgG. In: Journal of Immunology. 1996 ; Vol. 156, No. 8. pp. 2858-2865.
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