Abstract
The quantification of transcriptional variation in single cells, particularly within the same cell population, is currently limited by the low sensitivity and high technical noise of single-cell RNA-seq assays. We report multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq), a highly sensitive and quantitative method for single-cell sequencing of total RNA. By systematically determining technical noise, we show that MATQ-seq captures genuine biological variation between whole transcriptomes of single cells.
Original language | English (US) |
---|---|
Pages (from-to) | 267-270 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - Feb 28 2017 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology