TY - JOUR
T1 - Effects of ionizing radiation on telomere length and telomerase activity in cultured human lens epithelium cells
AU - Bains, Savneet Kaur
AU - Chapman, Kim
AU - Bright, Scott
AU - Senan, Anish
AU - Kadhim, Munira
AU - Slijepcevic, Predrag
N1 - Funding Information:
We would like to thank Dr Mark Hill, Mrs Deborah Bowler and Dr Terry Roberts for their help and support with this project. We would also like to thank European Commissioning, DoReMi for funding this project. This study was supported by the DoReMi Network of Excellence grant of the European Atomic Energy Community's 7th Framework Program (DoReMi, Low Dose Research towards Multidisciplinary Integration, work package 7. DoReMi Project grant agreement number 249689; http://www.doremi-noe.net/)
Publisher Copyright:
© 2018, Copyright © 2018 Taylor & Francis Group LLC.
PY - 2018/11/20
Y1 - 2018/11/20
N2 - Purpose: To investigate the effects of ionizing radiation on telomere length and telomerase activity in human lens epithelial cells. There are studies suggesting evidence of telomere length in association with opacity of the lens; however, these studies have been conducted on Canine Lens cells. Our study was designed to understand further the effects of different doses of ionizing radiation on telomere length and telomerase activity in cultured human lens epithelium cells from three Donors. Materials and methods: For this study, embryonic human lens epithelial (HLE) cells from three donors, obtained commercially were cultured. Telomere length and telomerase activity were measured after each passage until cells stopped growing in culture. This was repeated on irradiated (0.001 Gy, 0.01 Gy, 0.02 Gy, 0.1 Gy, 1 Gy and 2 Gy) cells. DNA damage response using the H2AX and telomere dysfunction foci assays were also examined at 30 mins, 24 hours, 48 hours and 72 hours postirradiation. Results and conclusion: We have demonstrated genetic changes in telomere length and oxidative stress, which may be relevant to cataractogenesis. Our study shows that in control cells telomere length increases as passage increases. We have also demonstrated that telomere length increases at higher doses of 1.0 Gy and 2.0 Gy. However, telomerase activity decreases dose dependently and as passages increase. These results are not conclusive and further studies ex vivo measuring lens opacity and telomere length in the model would be beneficial in a bigger cohort, hence confirming a link between telomere length, cataractogenesis and genetic factors.
AB - Purpose: To investigate the effects of ionizing radiation on telomere length and telomerase activity in human lens epithelial cells. There are studies suggesting evidence of telomere length in association with opacity of the lens; however, these studies have been conducted on Canine Lens cells. Our study was designed to understand further the effects of different doses of ionizing radiation on telomere length and telomerase activity in cultured human lens epithelium cells from three Donors. Materials and methods: For this study, embryonic human lens epithelial (HLE) cells from three donors, obtained commercially were cultured. Telomere length and telomerase activity were measured after each passage until cells stopped growing in culture. This was repeated on irradiated (0.001 Gy, 0.01 Gy, 0.02 Gy, 0.1 Gy, 1 Gy and 2 Gy) cells. DNA damage response using the H2AX and telomere dysfunction foci assays were also examined at 30 mins, 24 hours, 48 hours and 72 hours postirradiation. Results and conclusion: We have demonstrated genetic changes in telomere length and oxidative stress, which may be relevant to cataractogenesis. Our study shows that in control cells telomere length increases as passage increases. We have also demonstrated that telomere length increases at higher doses of 1.0 Gy and 2.0 Gy. However, telomerase activity decreases dose dependently and as passages increase. These results are not conclusive and further studies ex vivo measuring lens opacity and telomere length in the model would be beneficial in a bigger cohort, hence confirming a link between telomere length, cataractogenesis and genetic factors.
KW - cataracts
KW - DNA damage
KW - ionizing radiation
KW - oxidative stress
KW - telomerase
KW - Telomere
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U2 - 10.1080/09553002.2018.1466066
DO - 10.1080/09553002.2018.1466066
M3 - Article
C2 - 29667481
AN - SCOPUS:85046399437
SN - 0955-3002
VL - 95
SP - 54
EP - 63
JO - International journal of radiation biology
JF - International journal of radiation biology
IS - 1
ER -