Electroacupuncture reduces the expression of proinflammatory cytokines in inflamed skin tissues through activation of cannabinoid CB2 receptors.

T. F. Su, Y. Q. Zhao, L. H. Zhang, M. Peng, C. H. Wu, L. Pei, B. Tian, J. Zhang, J. Shi, Hui-Lin Pan, M. Li

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Endogenous cannabinoids and peripheral cannabinoid CB2 receptors (CB2Rs) are involved in the antinociceptive effect of electroacupuncture (EA) on inflammatory pain. However, it is not clear how CB2R activation contributes to the antinociceptive effect of EA. The major proinflammatory cytokines, such as tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6, are involved in inflammatory pain. Here we determined the effects of CB2R activation and EA on the expression level of IL-1β, IL-6 and TNF-α in inflamed skin tissues. Inflammatory pain was induced by injection of complete Freund's adjuvant into the left hindpaw of rats. Thermal hyperalgesia was tested with a radiant heat stimulus, and mechanical allodynia was quantified using von Frey filaments. The mRNA and protein levels of IL-1β, IL-6 and TNF-α in inflamed skin tissues were measured using real-time polymerase chain reaction and Western blot, respectively. Local injection of the selective CB2R agonist AM1241 or EA applied to GB30 and GB34 significantly reduced thermal hyperalgesia and mechanical allodynia induced by tissue inflammation. The specific CB2R antagonist AM630 significantly attenuated the antinociceptive effect of EA. Furthermore, EA or AM1241 treatment significantly decreased the mRNA and protein levels of IL-1β, IL-6 and TNF-α in inflamed skin tissues. In addition, pretreatment with AM630 significantly reversed the inhibitory effect of EA on these cytokine levels in inflamed skin tissues. Our results suggest that EA reduces inflammatory pain and proinflammatory cytokines in inflamed skin tissues through activation of CB2Rs.

Original languageEnglish (US)
Pages (from-to)624-635
Number of pages12
JournalEuropean journal of pain (London, England)
Volume16
Issue number5
StatePublished - Jan 1 2012

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Cannabinoid Receptor CB2
Electroacupuncture
Hyperalgesia
Cytokines
Skin
Interleukin-1
Interleukin-6
Tumor Necrosis Factor-alpha
Pain
Messenger RNA
Injections
Freund's Adjuvant
Cannabinoids
Real-Time Polymerase Chain Reaction
Proteins
Hot Temperature
Western Blotting
Inflammation

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Electroacupuncture reduces the expression of proinflammatory cytokines in inflamed skin tissues through activation of cannabinoid CB2 receptors. / Su, T. F.; Zhao, Y. Q.; Zhang, L. H.; Peng, M.; Wu, C. H.; Pei, L.; Tian, B.; Zhang, J.; Shi, J.; Pan, Hui-Lin; Li, M.

In: European journal of pain (London, England), Vol. 16, No. 5, 01.01.2012, p. 624-635.

Research output: Contribution to journalArticle

Su, T. F. ; Zhao, Y. Q. ; Zhang, L. H. ; Peng, M. ; Wu, C. H. ; Pei, L. ; Tian, B. ; Zhang, J. ; Shi, J. ; Pan, Hui-Lin ; Li, M. / Electroacupuncture reduces the expression of proinflammatory cytokines in inflamed skin tissues through activation of cannabinoid CB2 receptors. In: European journal of pain (London, England). 2012 ; Vol. 16, No. 5. pp. 624-635.
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