Abstract
Sections of paraffin embedded tissues are routinely used for studying tissue histology and histopathology. However, it is difficult to determine what the three-dimensional tissue morphology is from such sections. In addition, the sections of tissues examined may not contain the region within the tissue that is necessary for the purpose of the ongoing study. This latter limitation hinders histopathological studies of blood vessels since vascular lesions develop in a focalized manner. This requires a method that enables us to survey a wide area of the blood vessel wall, from its surface to deeper regions. A whole mount en face preparation of blood vessels fulfills this requirement. In this article, we will demonstrate how to make en face preparations of the mouse aorta and carotid artery and to immunofluorescently stain them for confocal microscopy and other types of fluorescence-based imaging.
Original language | English (US) |
---|---|
Article number | e55460 |
Journal | Journal of Visualized Experiments |
Volume | 2017 |
Issue number | 123 |
DOIs | |
State | Published - May 19 2017 |
Keywords
- Aorta
- Basic Protocol
- Carotid artery
- En face immunofluorescence preparation
- Endothelial cells
- Issue 123
ASJC Scopus subject areas
- General Neuroscience
- General Chemical Engineering
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology