En face preparation of mouse blood vessels

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sections of paraffin embedded tissues are routinely used for studying tissue histology and histopathology. However, it is difficult to determine what the three-dimensional tissue morphology is from such sections. In addition, the sections of tissues examined may not contain the region within the tissue that is necessary for the purpose of the ongoing study. This latter limitation hinders histopathological studies of blood vessels since vascular lesions develop in a focalized manner. This requires a method that enables us to survey a wide area of the blood vessel wall, from its surface to deeper regions. A whole mount en face preparation of blood vessels fulfills this requirement. In this article, we will demonstrate how to make en face preparations of the mouse aorta and carotid artery and to immunofluorescently stain them for confocal microscopy and other types of fluorescence-based imaging.

Original languageEnglish (US)
Article numbere55460
JournalJournal of Visualized Experiments
Volume2017
Issue number123
DOIs
StatePublished - May 19 2017

Fingerprint

Blood vessels
Blood Vessels
Tissue
Histology
Confocal microscopy
Optical Imaging
Carotid Arteries
Confocal Microscopy
Paraffin
Paraffins
Aorta
Coloring Agents
Fluorescence
Imaging techniques

Keywords

  • Aorta
  • Basic Protocol
  • Carotid artery
  • En face immunofluorescence preparation
  • Endothelial cells
  • Issue 123

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

En face preparation of mouse blood vessels. / Ko, Kyung Ae; Fujiwara, Keigi; Krishnan, Sunil; Abe, Jun Ichi.

In: Journal of Visualized Experiments, Vol. 2017, No. 123, e55460, 19.05.2017.

Research output: Contribution to journalArticle

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