@article{005d3ce577304f50849d899d5bd0c8f5,
title = "Engineered bone for probing organotypic growth and therapy response of prostate cancer tumoroids in vitro",
abstract = "Mechanistic analysis of metastatic prostate cancer (PCa) biology and therapy response critically depends upon clinically relevant three-dimensional (3D) bone-like, organotypic culture. We here combine an engineered bone-mimetic environment (BME) with longitudinal microscopy to test the growth and therapy response of 3D PCa tumoroids. Besides promoting both tumor-cell autonomous and microenvironment-dependent growth in PCa cell lines and patient-derived xenograft cells, the BME enables in vivo-like tumor cell response to therapy, and reveals bone stroma dependent resistance to chemotherapy and BME-targeted localization and induction of cytoxicity by Radium-223. The BME platform will allow the propagation, compound screening and mechanistic dissection of patient-derived bone tumor isolates and applications toward personalized medicine.",
keywords = "3D culture, Bone metastasis, In vitro engineered bone microenvironment, Microscopy, Prostate cancer, Therapy response",
author = "Claudia Paindelli and Nora Navone and Logothetis, {Christopher J.} and Peter Friedl and Eleonora Dondossola",
note = "Funding Information: We thank Prof. Dietmar W. Hutmacher and Dr. Elena M. De-Juan-Pardo for scaffold generation (Institute of Health and Biomedical Innovation, Queensland University, Brisbane, Australia); Peter Shepherd (the University of Texas, MD Anderson Cancer Center, Houston, TX, USA) for providing PDXs used for propagation. E.D. is supported by the Cancer Prevention and Research Institute of Texas (RP140482), TX, USA, the Prostate Cancer Foundation (16YOUN24), CA, USA and The Rolanette and Berdon Lawrence Bone Disease Program of Texas, TX, USA; P.F. is supported by NWO-VICI (918.11.626), the European Research Council (ERC-CoG DEEPINSIGHT, Project No. 617430), the Cancer Genomics Cancer, The Netherlands; E.D., P.F. and N.N are supported by the Prostate Cancer Moonshot Program at MD Anderson Cancer Center. The Genitourinary Cancers Program of the Cancer Center Support Grant (CCSG) shared resources at MD Anderson Cancer Center was supported by NIH/NCI award number P30 CA016672. Funding Information: We thank Prof. Dietmar W. Hutmacher and Dr. Elena M. De-Juan-Pardo for scaffold generation (Institute of Health and Biomedical Innovation, Queensland University, Brisbane, Australia); Peter Shepherd (the University of Texas, MD Anderson Cancer Center , Houston, TX, USA) for providing PDXs used for propagation. E.D. is supported by the Cancer Prevention and Research Institute of Texas (RP140482), TX, USA, the Prostate Cancer Foundation (16YOUN24), CA, USA and The Rolanette and Berdon Lawrence Bone Disease Program of Texas, TX, USA; P.F. is supported by NWO-VICI ( 918.11.626 ), the European Research Council (ERC-CoG DEEPINSIGHT, Project No. 617430 ), the Cancer Genomics Cancer, The Netherlands; E.D., P.F. and N.N are supported by the Prostate Cancer Moonshot Program at MD Anderson Cancer Center . The Genitourinary Cancers Program of the Cancer Center Support Grant (CCSG) shared resources at MD Anderson Cancer Center was supported by NIH / NCI award number P30 CA016672 . Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",
year = "2019",
month = mar,
doi = "10.1016/j.biomaterials.2019.01.027",
language = "English (US)",
volume = "197",
pages = "296--304",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
}