TY - JOUR
T1 - EphB-ephrin-B2 interactions are required for thymus migration during organogenesis
AU - Foster, Katie E.
AU - Gordon, Julie
AU - Cardenas, Kim
AU - Veiga-Fernandes, Henrique
AU - Makinen, Taija
AU - Grigorieva, Elena
AU - Wilkinson, David G.
AU - Clare Blackburng, C.
AU - Richie, Ellen
AU - Manley, Nancy R.
AU - Adams, Ralf H.
AU - Kioussis, Dimitris
AU - Coles, Mark C.
PY - 2010/7/27
Y1 - 2010/7/27
N2 - Thymus organogenesis requires coordinated interactions of multiple cell types, including neural crest (NC) cells, to orchestrate the formation, separation, and subsequent migration of the developing thymus from the third pharyngeal pouch to the thoracic cavity. The molecular mechanisms driving these processes are unclear; however, NC-derived mesenchyme has been shown to play an important role. Here, we show that, in the absence of ephrin-B2 expression on thymic NC-derived mesenchyme, the thymus remains in the cervical area instead of migrating into the thoracic cavity. Analysis of individual NC-derived thymic mesenchymal cells shows that, in the absence of ephrin-B2, their motility is impaired as a result of defective EphB receptor signaling. This implies a NC-derived cell-specific role of EphB-ephrin-B2 interactions in the collective migration of the thymic rudiment during organogenesis.
AB - Thymus organogenesis requires coordinated interactions of multiple cell types, including neural crest (NC) cells, to orchestrate the formation, separation, and subsequent migration of the developing thymus from the third pharyngeal pouch to the thoracic cavity. The molecular mechanisms driving these processes are unclear; however, NC-derived mesenchyme has been shown to play an important role. Here, we show that, in the absence of ephrin-B2 expression on thymic NC-derived mesenchyme, the thymus remains in the cervical area instead of migrating into the thoracic cavity. Analysis of individual NC-derived thymic mesenchymal cells shows that, in the absence of ephrin-B2, their motility is impaired as a result of defective EphB receptor signaling. This implies a NC-derived cell-specific role of EphB-ephrin-B2 interactions in the collective migration of the thymic rudiment during organogenesis.
KW - Collective cell migration
KW - Eph receptor
KW - Ephrin-B2
KW - Neural crest
KW - Thymus organogenesis
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UR - http://www.scopus.com/inward/citedby.url?scp=77955818628&partnerID=8YFLogxK
U2 - 10.1073/pnas.1003747107
DO - 10.1073/pnas.1003747107
M3 - Article
C2 - 20616004
AN - SCOPUS:77955818628
SN - 0027-8424
VL - 107
SP - 13414
EP - 13419
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 30
ER -