TY - JOUR
T1 - Epithelial morphogenesis in the perinatal mouse uterus
AU - Vue, Zer
AU - Behringer, Richard R.
N1 - Funding Information:
The authors thank Rachel D. Mullen and Shuo‐Ting Yen for their helpful discussions. The authors also thank Chih‐Wei Hsu, Tegy John Vadakkan, and Jason M. Kirk from the Baylor College of Medicine Optical Imaging and Vital Microscopy Core for microscopy and assistance, Adriana Paulucci and Henry Adams from the MD Anderson Department of Genetics Microscopy Core for microscopy, assistance, and postimage acquisition processing, Kenneth Trimmer for his help with the Imaris software, and the IDDRC Microscopy Core for their help with Imaris Filaments. These studies were supported by National Institutes of Health (NIH) grant HD030284 and the Ben F. Love Endowment to R. R. B. Z. V. was supported by NIH GM56929, NIH CA009299, and NIH HD30284. The Intellectual and Developmental Disabilities Research Center Microscopy Core was supported by NIH U54 HD083092 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development. Veterinary resources were supported by NIH grant CA16672.
Publisher Copyright:
© 2020 Wiley Periodicals LLC
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Background: The uterus is the location where multiple events occur that are required for the start of new life in mammals. The adult uterus contains endometrial or uterine glands that are essential for female fertility. In the mouse, uterine glands are located in the lateral and antimesometrial regions of the uterine horn. Previous three-dimensional (3D)-imaging of the adult uterus, its glands, and implanting embryos has been performed by multiple groups, using fluorescent microscopy. Adenogenesis, the formation of uterine glands, initiates after birth. Recently, we created a 3D-staging system of mouse uterine gland development at postnatal time points, using light sheet fluorescent microscopy. Here, using a similar approach, we examine the morphological changes in the epithelium of the perinatal mouse uterus. Results: The uterine epithelium exhibits dorsoventral (mesometrial-antimesometrial) patterning as early as 3 days after birth (P3), marked by the presence of the dorsally positioned developing uterine rail. Uterine gland buds are present beginning at P4. Novel morphological epithelial structures, including a ventral ridge and uterine segments were identified. Conclusions: The perinatal mouse uterine luminal epithelium develops dorsal-ventral morphologies at 3 to 4 days postpartum. Between 5 and 6 days postpartum uterine epithelial folds form, defining alternating left-right segments.
AB - Background: The uterus is the location where multiple events occur that are required for the start of new life in mammals. The adult uterus contains endometrial or uterine glands that are essential for female fertility. In the mouse, uterine glands are located in the lateral and antimesometrial regions of the uterine horn. Previous three-dimensional (3D)-imaging of the adult uterus, its glands, and implanting embryos has been performed by multiple groups, using fluorescent microscopy. Adenogenesis, the formation of uterine glands, initiates after birth. Recently, we created a 3D-staging system of mouse uterine gland development at postnatal time points, using light sheet fluorescent microscopy. Here, using a similar approach, we examine the morphological changes in the epithelium of the perinatal mouse uterus. Results: The uterine epithelium exhibits dorsoventral (mesometrial-antimesometrial) patterning as early as 3 days after birth (P3), marked by the presence of the dorsally positioned developing uterine rail. Uterine gland buds are present beginning at P4. Novel morphological epithelial structures, including a ventral ridge and uterine segments were identified. Conclusions: The perinatal mouse uterine luminal epithelium develops dorsal-ventral morphologies at 3 to 4 days postpartum. Between 5 and 6 days postpartum uterine epithelial folds form, defining alternating left-right segments.
KW - adenogenesis
KW - female reproductive tract
KW - postnatal development
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U2 - 10.1002/dvdy.234
DO - 10.1002/dvdy.234
M3 - Article
C2 - 32767478
AN - SCOPUS:85090122692
SN - 1058-8388
VL - 249
SP - 1377
EP - 1386
JO - Developmental Dynamics
JF - Developmental Dynamics
IS - 11
ER -