@article{a69204d85b004bb49e27d691e5c84108,
title = "Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung",
abstract = "Using imaging and single-cell RNA-seq, Vila Ellis et al. identify a lung endothelial cell (EC) population with a unique transcriptome, location, morphology, and function in lung development. These ECs are involved in alveolar morphogenesis independent of myofibroblasts.",
keywords = "CAR4, VEGF, alveologenesis, angiogenesis, endothelial cell, lung alveolar type 1 cell, lung development, lung vasculature, single-cell RNA-seq, vascular biology",
author = "{Vila Ellis}, Lisandra and Cain, {Margo P.} and Vera Hutchison and Per Flodby and Crandall, {Edward D.} and Zea Borok and Bin Zhou and Ostrin, {Edwin J.} and Wythe, {Joshua D.} and Jichao Chen",
note = "Funding Information: We thank Drs. Napoleone Ferrara (Genentech; currently University of California San Diego, USA), Ralf Adams (University of M{\"u}nster, Germany), and Brigid Hogan (Duke University, USA) for providing the VegfaCKO, Cdh5-CreER, and SftpcCreER mice, respectively. We thank Dr. Barry Starcher (University of Texas Health Science Center at Tyler) for the elastin antibody. We thank Kamryn Gerner-Mauro for assisting with scRNA-seq data analysis. The University of Texas M D Anderson Cancer Center DNA Analysis Facility and Flow Cytometry and Cellular Imaging Core Facility are supported by the Cancer Center Support Grant (CA #16672). This work was supported by the University of Texas M D Anderson Cancer Center Start-up Fund, an American Lung Association Innovation Award and National Institutes of Health R01HL130129 (J.C.), and National Institutes of Health R35HL135747 (Z.B.). L.V.E. J.D.W. and J.C. designed the research; L.V.E. M.P.C. V.H. E.J.O. and J.C. performed the research; P.F. E.D.C. and Z.B. provided the Aqp5Cre mice; B.Z. provided the AplnCreER mice; L.V.E. J.D.W. and J.C. wrote the paper; and all authors read and approved the paper. The authors declare no competing interests. Funding Information: We thank Drs. Napoleone Ferrara (Genentech; currently University of California San Diego, USA), Ralf Adams (University of M{\"u}nster, Germany), and Brigid Hogan (Duke University, USA) for providing the Vegfa CKO , Cdh5-CreER, and Sftpc CreER mice, respectively. We thank Dr. Barry Starcher (University of Texas Health Science Center at Tyler) for the elastin antibody. We thank Kamryn Gerner-Mauro for assisting with scRNA-seq data analysis. The University of Texas M D Anderson Cancer Center DNA Analysis Facility and Flow Cytometry and Cellular Imaging Core Facility are supported by the Cancer Center Support Grant (CA # 16672 ). This work was supported by the University of Texas M D Anderson Cancer Center Start-up Fund, an American Lung Association Innovation Award and National Institutes of Health R01HL130129 (J.C.), and National Institutes of Health R35HL135747 (Z.B.). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = mar,
day = "9",
doi = "10.1016/j.devcel.2020.01.009",
language = "English (US)",
volume = "52",
pages = "617--630.e6",
journal = "Developmental cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",
}