Epstein-Barr virus particles induce centrosome amplification and chromosomal instability

Anatoliy Shumilov, Ming Han Tsai, Yvonne T. Schlosser, Anne Sophie Kratz, Katharina Bernhardt, Susanne Fink, Tuba Mizani, Xiaochen Lin, Anna Jauch, Josef Mautner, Annette Kopp-Schneider, Regina Feederle, Ingrid Hoffmann, Henri Jacques Delecluse

    Research output: Contribution to journalArticle

    27 Scopus citations

    Abstract

    Infections with Epstein-Barr virus (EBV) are associated with cancer development, and EBV lytic replication (the process that generates virus progeny) is a strong risk factor for some cancer types. Here we report that EBV infection of B-lymphocytes (in vitro and in a mouse model) leads to an increased rate of centrosome amplification, associated with chromosomal instability. This effect can be reproduced with virus-like particles devoid of EBV DNA, but not with defective virus-like particles that cannot infect host cells. Viral protein BNRF1 induces centrosome amplification, and BNRF1-deficient viruses largely lose this property. These findings identify a new mechanism by which EBV particles can induce chromosomal instability without establishing a chronic infection, thereby conferring a risk for development of tumours that do not necessarily carry the viral genome.

    Original languageEnglish (US)
    Article number14257
    JournalNature communications
    Volume8
    DOIs
    StatePublished - Feb 10 2017

    ASJC Scopus subject areas

    • Chemistry(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Physics and Astronomy(all)

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  • Cite this

    Shumilov, A., Tsai, M. H., Schlosser, Y. T., Kratz, A. S., Bernhardt, K., Fink, S., Mizani, T., Lin, X., Jauch, A., Mautner, J., Kopp-Schneider, A., Feederle, R., Hoffmann, I., & Delecluse, H. J. (2017). Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nature communications, 8, [14257]. https://doi.org/10.1038/ncomms14257