Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In vitro drug combination studies are commonly used for CLL primary lymphocytes. An advancement in this method is to perform ex vivo drug testing where the first agent is administered to patients and second drug is tested in these patients’ cells in vitro. These assays have been effective in identifying novel agents that work additively or synergistically. In this chapter, we provide a step-by-step protocol for ex vivo drug testing that can be used for combination strategies.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-25
Number of pages7
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1881
ISSN (Print)1064-3745

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Pharmacology
Pharmaceutical Preparations
Drug Combinations
Lymphocytes
In Vitro Techniques

Keywords

  • Annexin V-FITC/PI staining
  • Apoptosis
  • CLL
  • Combination therapy
  • Ex vivo drug testing
  • Flow cytometry
  • Targeted therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Aslan, B., Ayres, M. L., & Gandhi, V. V. (2019). Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells. In Methods in Molecular Biology (pp. 19-25). (Methods in Molecular Biology; Vol. 1881). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8876-1_2

Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells. / Aslan, Burcu; Ayres, Mary L.; Gandhi, Varsha V.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 19-25 (Methods in Molecular Biology; Vol. 1881).

Research output: Chapter in Book/Report/Conference proceedingChapter

Aslan, B, Ayres, ML & Gandhi, VV 2019, Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1881, Humana Press Inc., pp. 19-25. https://doi.org/10.1007/978-1-4939-8876-1_2
Aslan B, Ayres ML, Gandhi VV. Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 19-25. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8876-1_2
Aslan, Burcu ; Ayres, Mary L. ; Gandhi, Varsha V. / Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 19-25 (Methods in Molecular Biology).
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