@inbook{33ea27be01bf47089b05a841d64a4a07,
title = "Exosomes from cell culture-conditioned medium: Isolation by ultracentrifugation and characterization",
abstract = "Exosomes are small vesicles of endosomal origin secreted by most cell types. Recent studies have identified exosomes as important mediators of intercellular communication and as important source materials for many clinical applications, including minimal invasive disease diagnosis. Exosomes have been purified from in vitro cell culture supernatants by many different methods; however the most simple and reliable method involves purification by ultracentrifugation. This chapter describes a detailed protocol for isolating exosomes from cell culture-conditioned medium using ultracentrifugation and their characterization based upon size, number, and protein expression by several complementary methods such as transmission electron microscopy, nanoparticle tracking analysis, western blotting, and flow cytometry.",
keywords = "Cell culture, Conditioned medium, Exosomes, Flow cytometry, Sucrose, Transmission electron microscopy, Ultracentrifugation, Western blot",
author = "Anurag Purushothaman",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9133-4_19",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "233--244",
booktitle = "Methods in Molecular Biology",
}