Experimental approaches to investigate the role of helicase acetylation in regulating r-loop stability

Chenlin Song, Ingrid Grummt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

R-loops are three-stranded nucleic acid structures composed of a DNA-RNA heteroduplex and a displaced single-stranded DNA. Although R-loops serve important roles in transcription and chromatin structure, they are also a major threat to genome stability. Cells prevent accumulation of genomic R-loops by mechanisms that remove these structures, such as ribonucleases which digest DNA-RNA hybrids and helicases which unwind R-loops. Here we describe methods to monitor resolvement of R-loops by the helicase DDX21 focussing on the impact of acetylation on helicase activity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages237-253
Number of pages17
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1983
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Nucleic Acid Heteroduplexes
RNA Helicases
Genomic Instability
Single-Stranded DNA
Acetylation
Ribonucleases
Nucleic Acids
Chromatin
RNA
DNA

Keywords

  • DDX21
  • DNA-RNA hybrids
  • Gel electrophoresis
  • Helicase
  • Immunopurification
  • Protein acetylation
  • R-loops
  • Recombinant proteins
  • Unwinding of DNA-RNA structures

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Song, C., & Grummt, I. (2019). Experimental approaches to investigate the role of helicase acetylation in regulating r-loop stability. In Methods in Molecular Biology (pp. 237-253). (Methods in Molecular Biology; Vol. 1983). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9434-2_14

Experimental approaches to investigate the role of helicase acetylation in regulating r-loop stability. / Song, Chenlin; Grummt, Ingrid.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 237-253 (Methods in Molecular Biology; Vol. 1983).

Research output: Chapter in Book/Report/Conference proceedingChapter

Song, C & Grummt, I 2019, Experimental approaches to investigate the role of helicase acetylation in regulating r-loop stability. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1983, Humana Press Inc., pp. 237-253. https://doi.org/10.1007/978-1-4939-9434-2_14
Song C, Grummt I. Experimental approaches to investigate the role of helicase acetylation in regulating r-loop stability. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 237-253. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9434-2_14
Song, Chenlin ; Grummt, Ingrid. / Experimental approaches to investigate the role of helicase acetylation in regulating r-loop stability. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 237-253 (Methods in Molecular Biology).
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